1nof
From Proteopedia
(New page: 200px<br /> <applet load="1nof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nof, resolution 1.42Å" /> '''THE FIRST CRYSTALLO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1NOF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]]. | + | 1NOF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]]. |
==Reference== | ==Reference== | ||
First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis., Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A, Biochemistry. 2003 Jul 22;42(28):8411-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12859186 12859186] | First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis., Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A, Biochemistry. 2003 Jul 22;42(28):8411-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12859186 12859186] | ||
| + | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Erwinia chrysanthemi]] | [[Category: Erwinia chrysanthemi]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:19:08 2007'' |
Revision as of 12:14, 30 October 2007
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THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS
Overview
The room-temperature structure of xylanase (EC 3.2.1.8) from the bacterial, plant pathogen Erwinia chrysanthemi expressed in Escherichia coli, a 45, kDa, 413-amino acid protein belonging to glycoside hydrolase family 5, has, been determined by multiple isomorphous replacement and refined to a, resolution of 1.42 A. This represents the first structure of a xylanase, not belonging to either glycoside hydrolase family 10 or family 11. The, enzyme is composed of two domains similar to most family 10 xylanases and, the alpha-amylases. The catalytic domain (residues 46-315) has a, (beta/alpha)(8)-barrel motif with a binding cleft along the C-terminal, side of the beta-barrel. The catalytic residues, Glu165 and Glu253, determined by correspondence to other family 5 and family 10 glycoside, ... [(full description)]
About this Structure
1NOF is a [Single protein] structure of sequence from [Erwinia chrysanthemi] with ACT as [ligand]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [OCA].
Reference
First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis., Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A, Biochemistry. 2003 Jul 22;42(28):8411-22. PMID:12859186
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