1idf
From Proteopedia
(New page: 200px<br /><applet load="1idf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1idf, resolution 2.5Å" /> '''ISOCITRATE DEHYDROGEN...) |
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| - | [[Image:1idf.jpg|left|200px]]<br /><applet load="1idf" size=" | + | [[Image:1idf.jpg|left|200px]]<br /><applet load="1idf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1idf, resolution 2.5Å" /> | caption="1idf, resolution 2.5Å" /> | ||
'''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''<br /> | '''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution | + | Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized. |
==About this Structure== | ==About this Structure== | ||
| - | 1IDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http:// | + | 1IDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Isocitrate dehydrogenase (NADP(+))]] | [[Category: Isocitrate dehydrogenase (NADP(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bolduc, J | + | [[Category: Bolduc, J M.]] |
| - | [[Category: Dyer, D | + | [[Category: Dyer, D H.]] |
| - | [[Category: Junior, D | + | [[Category: Junior, D E.Koshland.]] |
| - | [[Category: Scott, W | + | [[Category: Scott, W G.]] |
[[Category: Singer, P.]] | [[Category: Singer, P.]] | ||
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
| - | [[Category: Sweet, R | + | [[Category: Sweet, R M.]] |
[[Category: oxidoreductase (nad(a)-choh(d))]] | [[Category: oxidoreductase (nad(a)-choh(d))]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:44 2008'' |
Revision as of 11:10, 21 February 2008
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ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME
Overview
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.
About this Structure
1IDF is a Single protein structure of sequence from Escherichia coli. Active as Isocitrate dehydrogenase (NADP(+)), with EC number 1.1.1.42 Full crystallographic information is available from OCA.
Reference
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:7761851
Page seeded by OCA on Thu Feb 21 13:10:44 2008
