1ier
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Horse-spleen apoferritin is known to crystallize in three different space | + | Horse-spleen apoferritin is known to crystallize in three different space groups, cubic F432, tetragonal P42(1)2 and orthorhombic P2(1)2(1)2. A structure comparison of the cubic and tetragonal forms is presented here. Both crystal forms were obtained by the vapor-diffusion technique and data were collected at 2.26 A (cubic crystal) and 2.60 A (tetragonal crystal) resolution. Two main differences were observed between these crystal structures: (i) whereas intermolecular contacts only involve salt-bridge type interactions via cadmium ions in the cubic structure, two types of interactions are observed in the tetragonal crystal (cadmium-ion-mediated salt bridges and hydrogen-bonding interactions) and (ii) cadmium ions bound in the threefold axes of ferritin molecules exhibit lower site-occupation factors in the tetragonal structure than in the cubic one. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dautant, A.]] | [[Category: Dautant, A.]] | ||
| - | [[Category: Estaintot, B | + | [[Category: Estaintot, B Langlois D.]] |
[[Category: Gallois, B.]] | [[Category: Gallois, B.]] | ||
[[Category: Granier, T.]] | [[Category: Granier, T.]] | ||
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[[Category: iron storage]] | [[Category: iron storage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:07 2008'' |
Revision as of 11:11, 21 February 2008
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CUBIC CRYSTAL STRUCTURE OF NATIVE HORSE SPLEEN FERRITIN
Overview
Horse-spleen apoferritin is known to crystallize in three different space groups, cubic F432, tetragonal P42(1)2 and orthorhombic P2(1)2(1)2. A structure comparison of the cubic and tetragonal forms is presented here. Both crystal forms were obtained by the vapor-diffusion technique and data were collected at 2.26 A (cubic crystal) and 2.60 A (tetragonal crystal) resolution. Two main differences were observed between these crystal structures: (i) whereas intermolecular contacts only involve salt-bridge type interactions via cadmium ions in the cubic structure, two types of interactions are observed in the tetragonal crystal (cadmium-ion-mediated salt bridges and hydrogen-bonding interactions) and (ii) cadmium ions bound in the threefold axes of ferritin molecules exhibit lower site-occupation factors in the tetragonal structure than in the cubic one.
About this Structure
1IER is a Single protein structure of sequence from Equus caballus with as ligand. Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Comparison of the structures of the cubic and tetragonal forms of horse-spleen apoferritin., Granier T, Gallois B, Dautant A, Langlois d'Estaintot B, Precigoux G, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):580-7. PMID:15299889
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