1ig9
From Proteopedia
(New page: 200px<br /><applet load="1ig9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ig9, resolution 2.60Å" /> '''Structure of the Rep...) |
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| - | [[Image:1ig9.gif|left|200px]]<br /><applet load="1ig9" size=" | + | [[Image:1ig9.gif|left|200px]]<br /><applet load="1ig9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ig9, resolution 2.60Å" /> | caption="1ig9, resolution 2.60Å" /> | ||
'''Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase'''<br /> | '''Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase | + | We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family. |
==About this Structure== | ==About this Structure== | ||
| - | 1IG9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18] with CA and TTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1IG9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=TTP:'>TTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage rb18]] | [[Category: Enterobacteria phage rb18]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Franklin, M | + | [[Category: Franklin, M C.]] |
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: two metal ion mechanism]] | [[Category: two metal ion mechanism]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:35 2008'' |
Revision as of 11:11, 21 February 2008
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Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase
Overview
We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.
About this Structure
1IG9 is a Single protein structure of sequence from Enterobacteria phage rb18 with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:11389835
Page seeded by OCA on Thu Feb 21 13:11:35 2008
