1igj
From Proteopedia
(New page: 200px<br /> <applet load="1igj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1igj, resolution 2.5Å" /> '''26-10 FAB:DIGOXIN CO...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1igj.gif|left|200px]]<br /> | + | [[Image:1igj.gif|left|200px]]<br /><applet load="1igj" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1igj" size=" | + | |
caption="1igj, resolution 2.5Å" /> | caption="1igj, resolution 2.5Å" /> | ||
'''26-10 FAB:DIGOXIN COMPLEX-AFFINITY AND SPECIFICITY DUE TO SURFACE COMPLEMENTARITY'''<br /> | '''26-10 FAB:DIGOXIN COMPLEX-AFFINITY AND SPECIFICITY DUE TO SURFACE COMPLEMENTARITY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the three-dimensional structures of the antigen-binding | + | We have determined the three-dimensional structures of the antigen-binding fragment of the anti-digoxin monoclonal antibody 26-10 in the uncomplexed state at 2.7 A resolution and as a complex with digoxin at 2.5 A resolution. Neither the antibody nor digoxin undergoes any significant conformational changes upon forming the complex. Digoxin interacts primarily with the antibody heavy chain and is oriented such that the carbohydrate groups are exposed to solvent and the lactone ring is buried in a deep pocket at the bottom of the combining site. Despite extensive interactions between antibody and antigen, no hydrogen bonds or salt links are formed between 26-10 and digoxin. Thus the 26-10-digoxin complex is unique among the known three-dimensional structures of antibody-antigen complexes in that specificity and high affinity arise primarily from shape complementarity. |
==About this Structure== | ==About this Structure== | ||
| - | 1IGJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with DGX as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IGJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=DGX:'>DGX</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGJ OCA]. |
==Reference== | ==Reference== | ||
26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity., Jeffrey PD, Strong RK, Sieker LC, Chang CY, Campbell RL, Petsko GA, Haber E, Margolies MN, Sheriff S, Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10310-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8234291 8234291] | 26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity., Jeffrey PD, Strong RK, Sieker LC, Chang CY, Campbell RL, Petsko GA, Haber E, Margolies MN, Sheriff S, Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10310-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8234291 8234291] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Jeffrey, P | + | [[Category: Jeffrey, P D.]] |
[[Category: Sheriff, S.]] | [[Category: Sheriff, S.]] | ||
[[Category: DGX]] | [[Category: DGX]] | ||
[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:37 2008'' |
Revision as of 11:11, 21 February 2008
|
26-10 FAB:DIGOXIN COMPLEX-AFFINITY AND SPECIFICITY DUE TO SURFACE COMPLEMENTARITY
Overview
We have determined the three-dimensional structures of the antigen-binding fragment of the anti-digoxin monoclonal antibody 26-10 in the uncomplexed state at 2.7 A resolution and as a complex with digoxin at 2.5 A resolution. Neither the antibody nor digoxin undergoes any significant conformational changes upon forming the complex. Digoxin interacts primarily with the antibody heavy chain and is oriented such that the carbohydrate groups are exposed to solvent and the lactone ring is buried in a deep pocket at the bottom of the combining site. Despite extensive interactions between antibody and antigen, no hydrogen bonds or salt links are formed between 26-10 and digoxin. Thus the 26-10-digoxin complex is unique among the known three-dimensional structures of antibody-antigen complexes in that specificity and high affinity arise primarily from shape complementarity.
About this Structure
1IGJ is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity., Jeffrey PD, Strong RK, Sieker LC, Chang CY, Campbell RL, Petsko GA, Haber E, Margolies MN, Sheriff S, Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10310-4. PMID:8234291
Page seeded by OCA on Thu Feb 21 13:11:37 2008
