1ihf
From Proteopedia
(New page: 200px<br /><applet load="1ihf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ihf, resolution 2.200Å" /> '''INTEGRATION HOST FA...) |
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| - | [[Image:1ihf.gif|left|200px]]<br /><applet load="1ihf" size=" | + | [[Image:1ihf.gif|left|200px]]<br /><applet load="1ihf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ihf, resolution 2.200Å" /> | caption="1ihf, resolution 2.200Å" /> | ||
'''INTEGRATION HOST FACTOR/DNA COMPLEX'''<br /> | '''INTEGRATION HOST FACTOR/DNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Integration host factor (IHF) is a small heterodimeric protein that | + | Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a proline residue. IHF contacts the DNA exclusively via the phosphodiester backbone and the minor groove and relies heavily on indirect readout to recognize its binding sequence. One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove. |
==About this Structure== | ==About this Structure== | ||
| - | 1IHF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IHF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mizuuchi, K.]] | [[Category: Mizuuchi, K.]] | ||
| - | [[Category: Nash, H | + | [[Category: Nash, H A.]] |
| - | [[Category: Rice, P | + | [[Category: Rice, P A.]] |
| - | [[Category: Yang, S | + | [[Category: Yang, S W.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: beta ribbon motif]] | [[Category: beta ribbon motif]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:51 2008'' |
Revision as of 11:11, 21 February 2008
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INTEGRATION HOST FACTOR/DNA COMPLEX
Overview
Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a proline residue. IHF contacts the DNA exclusively via the phosphodiester backbone and the minor groove and relies heavily on indirect readout to recognize its binding sequence. One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove.
About this Structure
1IHF is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn., Rice PA, Yang S, Mizuuchi K, Nash HA, Cell. 1996 Dec 27;87(7):1295-306. PMID:8980235
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