1ik1
From Proteopedia
(New page: 200px<br /><applet load="1ik1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ik1" /> '''Solution Structure of an RNA Hairpin from HR...) |
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| - | [[Image:1ik1.gif|left|200px]]<br /><applet load="1ik1" size=" | + | [[Image:1ik1.gif|left|200px]]<br /><applet load="1ik1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ik1" /> | caption="1ik1" /> | ||
'''Solution Structure of an RNA Hairpin from HRV-14'''<br /> | '''Solution Structure of an RNA Hairpin from HRV-14'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 5' noncoding region of the picornaviral genome begins with a | + | The 5' noncoding region of the picornaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem-loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop. |
==About this Structure== | ==About this Structure== | ||
| - | 1IK1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1IK1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dutta, K.]] | [[Category: Dutta, K.]] | ||
[[Category: Huang, H.]] | [[Category: Huang, H.]] | ||
| - | [[Category: III, T | + | [[Category: III, T W.Barnes.]] |
| - | [[Category: Pascal, S | + | [[Category: Pascal, S M.]] |
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
[[Category: a form helix]] | [[Category: a form helix]] | ||
[[Category: triloop]] | [[Category: triloop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:41 2008'' |
Revision as of 11:12, 21 February 2008
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Solution Structure of an RNA Hairpin from HRV-14
Overview
The 5' noncoding region of the picornaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem-loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop.
About this Structure
1IK1 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structure of an RNA hairpin from HRV-14., Huang H, Alexandrov A, Chen X, Barnes TW 3rd, Zhang H, Dutta K, Pascal SM, Biochemistry. 2001 Jul 10;40(27):8055-64. PMID:11434774
Page seeded by OCA on Thu Feb 21 13:12:41 2008
