1imw
From Proteopedia
(New page: 200px<br /><applet load="1imw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1imw" /> '''Peptide Antagonist of IGFBP-1'''<br /> ==Ov...) |
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| - | [[Image:1imw.gif|left|200px]]<br /><applet load="1imw" size=" | + | [[Image:1imw.gif|left|200px]]<br /><applet load="1imw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1imw" /> | caption="1imw" /> | ||
'''Peptide Antagonist of IGFBP-1'''<br /> | '''Peptide Antagonist of IGFBP-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Highly structured, peptide antagonists of the interaction between | + | Highly structured, peptide antagonists of the interaction between insulin-like growth factor 1 (IGF-I) and IGF binding protein 1 (IGFBP-1) have recently been discovered by phage display of naive peptide libraries [Lowman, H. B., et al. (1998) Biochemistry 37, 8870--8878]. We now report a detailed analysis of the features of this turn-helix peptide motif that are necessary for IGFBP-1 binding and structural integrity. Further rounds of phage randomization indicate the importance of residues contributing to a hydrophobic patch on one face of the helix. Alanine-scanning substitutions confirm that the hydrophobic residues are necessary for binding. However, structural analysis by NMR spectroscopy indicates that some of these analogues are less well folded. Structured, high-affinity analogues that lack the disulfide bond were prepared by introducing a covalent constraint between side chains at positions i and i + 7 or i + 8 within the helix. Analogues based on this scaffold demonstrate that a helical conformation is present in the bound state, and that hydrophobic side chains in this helix, and residues immediately preceding it, interact with IGFBP-1. By comparison of alanine scanning data for IGF-I and the turn-helix peptide, we propose a model for common surface features of these molecules that recognize IGFBP-1. |
==About this Structure== | ==About this Structure== | ||
| - | 1IMW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IMW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMW OCA]. |
==Reference== | ==Reference== | ||
Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11456486 11456486] | Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11456486 11456486] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chen, Y | + | [[Category: Chen, Y M.]] |
| - | [[Category: Clark, R | + | [[Category: Clark, R G.]] |
| - | [[Category: Lowman, H | + | [[Category: Lowman, H B.]] |
| - | [[Category: Mortensen, D | + | [[Category: Mortensen, D L.]] |
| - | [[Category: Robinson, I | + | [[Category: Robinson, I C.]] |
| - | [[Category: Sadick, M | + | [[Category: Sadick, M D.]] |
| - | [[Category: Skelton, N | + | [[Category: Skelton, N J.]] |
| - | [[Category: Tomlinson, E | + | [[Category: Tomlinson, E E.]] |
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: loop-turn-helix]] | [[Category: loop-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:13:27 2008'' |
Revision as of 11:13, 21 February 2008
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Peptide Antagonist of IGFBP-1
Overview
Highly structured, peptide antagonists of the interaction between insulin-like growth factor 1 (IGF-I) and IGF binding protein 1 (IGFBP-1) have recently been discovered by phage display of naive peptide libraries [Lowman, H. B., et al. (1998) Biochemistry 37, 8870--8878]. We now report a detailed analysis of the features of this turn-helix peptide motif that are necessary for IGFBP-1 binding and structural integrity. Further rounds of phage randomization indicate the importance of residues contributing to a hydrophobic patch on one face of the helix. Alanine-scanning substitutions confirm that the hydrophobic residues are necessary for binding. However, structural analysis by NMR spectroscopy indicates that some of these analogues are less well folded. Structured, high-affinity analogues that lack the disulfide bond were prepared by introducing a covalent constraint between side chains at positions i and i + 7 or i + 8 within the helix. Analogues based on this scaffold demonstrate that a helical conformation is present in the bound state, and that hydrophobic side chains in this helix, and residues immediately preceding it, interact with IGFBP-1. By comparison of alanine scanning data for IGF-I and the turn-helix peptide, we propose a model for common surface features of these molecules that recognize IGFBP-1.
About this Structure
1IMW is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:11456486
Page seeded by OCA on Thu Feb 21 13:13:27 2008
