1io5
From Proteopedia
(New page: 200px<br /><applet load="1io5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1io5, resolution 2.00Å" /> '''HYDROGEN AND HYDRATI...) |
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| - | [[Image:1io5.gif|left|200px]]<br /><applet load="1io5" size=" | + | [[Image:1io5.gif|left|200px]]<br /><applet load="1io5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1io5, resolution 2.00Å" /> | caption="1io5, resolution 2.00Å" /> | ||
'''HYDROGEN AND HYDRATION OF HEN EGG-WHITE LYSOZYME DETERMINED BY NEUTRON DIFFRACTION'''<br /> | '''HYDROGEN AND HYDRATION OF HEN EGG-WHITE LYSOZYME DETERMINED BY NEUTRON DIFFRACTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Neutron quasi-Laue diffraction data (2 A resolution) from tetragonal hen | + | Neutron quasi-Laue diffraction data (2 A resolution) from tetragonal hen egg-white lysozyme were collected in ten days with neutron imaging plates. The data processing Laue software, LAUEGEN, developed for X-ray Laue diffractometry, was adapted for neutron diffractometry with a cylindrical detector. The data analysis software, X-PLOR, was modified and used for the refinement of hydrogen atoms, and the positions of 960 hydrogen atoms in the protein and 157 bound water molecules, were determined. Several examples are given of the methods used to identify hydrogen atoms and water molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1IO5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with DOD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1IO5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IO5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Castagna, J | + | [[Category: Castagna, J C.]] |
[[Category: Cipriani, F.]] | [[Category: Cipriani, F.]] | ||
[[Category: Hoeghoej, P.]] | [[Category: Hoeghoej, P.]] | ||
| - | [[Category: Lehmann, M | + | [[Category: Lehmann, M S.]] |
[[Category: Minezaki, Y.]] | [[Category: Minezaki, Y.]] | ||
[[Category: Niimura, N.]] | [[Category: Niimura, N.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:13:49 2008'' |
Revision as of 11:13, 21 February 2008
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HYDROGEN AND HYDRATION OF HEN EGG-WHITE LYSOZYME DETERMINED BY NEUTRON DIFFRACTION
Overview
Neutron quasi-Laue diffraction data (2 A resolution) from tetragonal hen egg-white lysozyme were collected in ten days with neutron imaging plates. The data processing Laue software, LAUEGEN, developed for X-ray Laue diffractometry, was adapted for neutron diffractometry with a cylindrical detector. The data analysis software, X-PLOR, was modified and used for the refinement of hydrogen atoms, and the positions of 960 hydrogen atoms in the protein and 157 bound water molecules, were determined. Several examples are given of the methods used to identify hydrogen atoms and water molecules.
About this Structure
1IO5 is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Neutron Laue diffractometry with an imaging plate provides an effective data collection regime for neutron protein crystallography., Niimura N, Minezaki Y, Nonaka T, Castagna JC, Cipriani F, Hoghoj P, Lehmann MS, Wilkinson C, Nat Struct Biol. 1997 Nov;4(11):909-14. PMID:9360606
Page seeded by OCA on Thu Feb 21 13:13:49 2008
Categories: Gallus gallus | Lysozyme | Single protein | Castagna, J C. | Cipriani, F. | Hoeghoej, P. | Lehmann, M S. | Minezaki, Y. | Niimura, N. | Nonaka, T. | Wilkinson, C. | DOD | Hydration | Hydrogen | Hydrolase
