1iqo
From Proteopedia
(New page: 200px<br /><applet load="1iqo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iqo" /> '''Solution structure of MTH1880 from methanoba...) |
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| - | [[Image:1iqo.jpg|left|200px]]<br /><applet load="1iqo" size=" | + | [[Image:1iqo.jpg|left|200px]]<br /><applet load="1iqo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1iqo" /> | caption="1iqo" /> | ||
'''Solution structure of MTH1880 from methanobacterium thermoautotrophicum'''<br /> | '''Solution structure of MTH1880 from methanobacterium thermoautotrophicum'''<br /> | ||
==Overview== | ==Overview== | ||
| - | MTH1880 is a hypothetical protein from Methanobacterium | + | MTH1880 is a hypothetical protein from Methanobacterium thermoautotrophicum, a target organism of structural genomics. The solution structure determined by NMR spectroscopy demonstrates a typical alpha + beta-fold found in many proteins with different functions. The molecular surface of the protein reveals a small, highly acidic pocket comprising loop B (Asp36, Asp37, Asp38), the end of beta2 (Glu39), and loop D (Ser57, Ser58, Ser61), indicating that the protein would have a possible cation binding site. The NMR resonances of several amino acids within the acidic binding pocket in MTH1880, shifted upon addition of calcium ion. This calcium binding motif and overall topology of MTH1880 differ from those of other calcium binding proteins. MTH1880 did not show a calcium-induced conformational change typical of calcium sensor proteins. Therefore, we propose that the MTH1880 protein contains a novel motif for calcium-specific binding, and may function as a calcium buffering protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1IQO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter Methanothermobacter]. Full crystallographic information is available from [http:// | + | 1IQO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter Methanothermobacter]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IQO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanothermobacter]] | [[Category: Methanothermobacter]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
[[Category: Bang, E.]] | [[Category: Bang, E.]] | ||
| - | [[Category: Jung, J | + | [[Category: Jung, J W.]] |
| - | [[Category: Lee, C | + | [[Category: Lee, C H.]] |
[[Category: Lee, W.]] | [[Category: Lee, W.]] | ||
[[Category: Shin, J.]] | [[Category: Shin, J.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:32 2008'' |
Revision as of 11:14, 21 February 2008
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Solution structure of MTH1880 from methanobacterium thermoautotrophicum
Overview
MTH1880 is a hypothetical protein from Methanobacterium thermoautotrophicum, a target organism of structural genomics. The solution structure determined by NMR spectroscopy demonstrates a typical alpha + beta-fold found in many proteins with different functions. The molecular surface of the protein reveals a small, highly acidic pocket comprising loop B (Asp36, Asp37, Asp38), the end of beta2 (Glu39), and loop D (Ser57, Ser58, Ser61), indicating that the protein would have a possible cation binding site. The NMR resonances of several amino acids within the acidic binding pocket in MTH1880, shifted upon addition of calcium ion. This calcium binding motif and overall topology of MTH1880 differ from those of other calcium binding proteins. MTH1880 did not show a calcium-induced conformational change typical of calcium sensor proteins. Therefore, we propose that the MTH1880 protein contains a novel motif for calcium-specific binding, and may function as a calcium buffering protein.
About this Structure
1IQO is a Single protein structure of sequence from Methanothermobacter. Full crystallographic information is available from OCA.
Reference
Solution structure of a novel calcium binding protein, MTH1880, from Methanobacterium thermoautotrophicum., Lee CH, Jung JW, Yee A, Arrowsmith CH, Lee W, Protein Sci. 2004 Apr;13(4):1148-54. PMID:15044740
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