1iqp
From Proteopedia
(New page: 200px<br /><applet load="1iqp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iqp, resolution 2.8Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1iqp.gif|left|200px]]<br /><applet load="1iqp" size=" | + | [[Image:1iqp.gif|left|200px]]<br /><applet load="1iqp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1iqp, resolution 2.8Å" /> | caption="1iqp, resolution 2.8Å" /> | ||
'''Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus'''<br /> | '''Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In eukaryotic DNA replication, replication factor-C (RFC) acts as the | + | In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes. |
==About this Structure== | ==About this Structure== | ||
| - | 1IQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IQP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cann, I | + | [[Category: Cann, I K.O.]] |
[[Category: Ishino, S.]] | [[Category: Ishino, S.]] | ||
[[Category: Ishino, Y.]] | [[Category: Ishino, Y.]] | ||
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[[Category: extended aaa-atpase domain]] | [[Category: extended aaa-atpase domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:34 2008'' |
Revision as of 11:14, 21 February 2008
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Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus
Overview
In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.
About this Structure
1IQP is a Single protein structure of sequence from Pyrococcus furiosus with as ligand. Full crystallographic information is available from OCA.
Reference
Atomic structure of the clamp loader small subunit from Pyrococcus furiosus., Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K, Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747
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