1ir6
From Proteopedia
(New page: 200px<br /><applet load="1ir6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ir6, resolution 2.90Å" /> '''Crystal structure of...) |
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| - | [[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size=" | + | [[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ir6, resolution 2.90Å" /> | caption="1ir6, resolution 2.90Å" /> | ||
'''Crystal structure of exonuclease RecJ bound to manganese'''<br /> | '''Crystal structure of exonuclease RecJ bound to manganese'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions | + | RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kakuta, Y.]] | [[Category: Kakuta, Y.]] | ||
[[Category: Masui, R.]] | [[Category: Masui, R.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Yamagata, A.]] | [[Category: Yamagata, A.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: two domains interconnected by alpha-helix]] | [[Category: two domains interconnected by alpha-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:51 2008'' |
Revision as of 11:14, 21 February 2008
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Crystal structure of exonuclease RecJ bound to manganese
Overview
RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA.
About this Structure
1IR6 is a Single protein structure of sequence from Thermus thermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity., Yamagata A, Kakuta Y, Masui R, Fukuyama K, Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5908-12. Epub 2002 Apr 23. PMID:11972066
Page seeded by OCA on Thu Feb 21 13:14:51 2008
Categories: Single protein | Thermus thermophilus | Fukuyama, K. | Kakuta, Y. | Masui, R. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yamagata, A. | MN | Dna recombination | Dna repair | Manganese | Nuclease | Riken structural genomics/proteomics initiative | Rsgi | Single-stranded dna | Structural genomics | Two domains interconnected by alpha-helix
