1irj
From Proteopedia
(New page: 200px<br /> <applet load="1irj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1irj, resolution 2.10Å" /> '''Crystal Structure o...) |
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| - | [[Image:1irj.gif|left|200px]]<br /> | + | [[Image:1irj.gif|left|200px]]<br /><applet load="1irj" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1irj" size=" | + | |
caption="1irj, resolution 2.10Å" /> | caption="1irj, resolution 2.10Å" /> | ||
'''Crystal Structure of the MRP14 complexed with CHAPS'''<br /> | '''Crystal Structure of the MRP14 complexed with CHAPS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Human MRP14 (hMRP14) is a Ca(2+)-binding protein from the S100 family of | + | Human MRP14 (hMRP14) is a Ca(2+)-binding protein from the S100 family of proteins. This protein is co-expressed with human MRP8 (hMRP8), a homologue protein in myeloid cells, and plays an indispensable role in Ca(2+)-dependent functions during inflammation. This role includes the activation of Mac-1, the beta(2) integrin which is involved in neutrophil adhesion to endothelial cells. The crystal structure of the holo form of hMRP14 was analyzed at 2.1 A resolution. hMRP14 is distinguished from other S100 member proteins by its long C-terminal region, and its structure shows that the region is extensively flexible. In this crystal structure of hMRP14, Chaps molecules bind to the hinge region that connects two EF-hand motifs, which suggests that this region is a target-binding site of this protein. Based on a structural comparison of hMRP14 with hMRP8 and human S100A12 (hS100A12) that is another homologue protein, the character of MRP8/14 hetero-complex and the functional significance of the flexibility of the C-terminal region of hMRP14 are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1IRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and CPS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1IRJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CPS:'>CPS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: s100a9]] | [[Category: s100a9]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:50 2008'' |
Revision as of 11:14, 21 February 2008
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Crystal Structure of the MRP14 complexed with CHAPS
Overview
Human MRP14 (hMRP14) is a Ca(2+)-binding protein from the S100 family of proteins. This protein is co-expressed with human MRP8 (hMRP8), a homologue protein in myeloid cells, and plays an indispensable role in Ca(2+)-dependent functions during inflammation. This role includes the activation of Mac-1, the beta(2) integrin which is involved in neutrophil adhesion to endothelial cells. The crystal structure of the holo form of hMRP14 was analyzed at 2.1 A resolution. hMRP14 is distinguished from other S100 member proteins by its long C-terminal region, and its structure shows that the region is extensively flexible. In this crystal structure of hMRP14, Chaps molecules bind to the hinge region that connects two EF-hand motifs, which suggests that this region is a target-binding site of this protein. Based on a structural comparison of hMRP14 with hMRP8 and human S100A12 (hS100A12) that is another homologue protein, the character of MRP8/14 hetero-complex and the functional significance of the flexibility of the C-terminal region of hMRP14 are discussed.
About this Structure
1IRJ is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of human MRP14 (S100A9), a Ca(2+)-dependent regulator protein in inflammatory process., Itou H, Yao M, Fujita I, Watanabe N, Suzuki M, Nishihira J, Tanaka I, J Mol Biol. 2002 Feb 15;316(2):265-76. PMID:11851337
Page seeded by OCA on Thu Feb 21 13:14:50 2008
Categories: Homo sapiens | Single protein | Itou, H. | Nishihira, J. | Tanaka, I. | Watanabe, N. | Yao, M. | CA | CPS | Calgranulin b | Ef-hand | Mrp14 | S100a9
