G18secL03Tpc4
From Proteopedia
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<Structure load='1ggq' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | <Structure load='1ggq' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | ||
| - | === | + | === '''Outer surface protein C (OspC) of ''Borrelia burgdorferi''''' === |
Outer surface protein C (OspC) is one of the major antigens on the surface of the Lyme disease spirochete, ''Borrelia burgdorferi'', along with other outer surface proteins A and B (OspA and OspB, respectively). It greatly differs from OspA and OspB in both structure and function. The uniqueness of OspC is that it comes into play when the pathogen is being transmitted to humans or other mammals. OspC is being produced by Borrelia burgdorferi during a very short time interval when infected ticks start feeding, but its synthesis is known to slow down greatly after transmission to a mammalian host. It was demonstrated that those spirochetes that lack OspC are capable to replicate inside and migrate to the salivary glands of the tick vector but do not infect mammals. <ref>D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [http://dx.doi.org/DOI:10.1093/emboj/20.5.971]</ref> Without OspC the spirochetes are believed to be unable to adapt to the environment inside the host. Therefore, OspC is believed to determine virulence of the spirochete to mammals, including humans. | Outer surface protein C (OspC) is one of the major antigens on the surface of the Lyme disease spirochete, ''Borrelia burgdorferi'', along with other outer surface proteins A and B (OspA and OspB, respectively). It greatly differs from OspA and OspB in both structure and function. The uniqueness of OspC is that it comes into play when the pathogen is being transmitted to humans or other mammals. OspC is being produced by Borrelia burgdorferi during a very short time interval when infected ticks start feeding, but its synthesis is known to slow down greatly after transmission to a mammalian host. It was demonstrated that those spirochetes that lack OspC are capable to replicate inside and migrate to the salivary glands of the tick vector but do not infect mammals. <ref>D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [http://dx.doi.org/DOI:10.1093/emboj/20.5.971]</ref> Without OspC the spirochetes are believed to be unable to adapt to the environment inside the host. Therefore, OspC is believed to determine virulence of the spirochete to mammals, including humans. | ||
| - | + | === Basic Structure Description === | |
| - | + | The ospC gene is located on a 27 kb circular plasmid and encodes a lipoprotein of 22–23 kDa.<ref>PMID:7679385</ref> The protein is initially synthesized with an 18-amino-acid-long signal sequence which is removed during processing and lipidation at the amino proximal Cys residue. OspC proteins are highly polymorphic and this variability extends even to strains collected from a single geographical area.<ref>D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [http://dx.doi.org/DOI:10.1093/emboj/20.5.971]</ref> | |
| - | === Basic Structure === | + | * |
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=== Main Function === | === Main Function === | ||
==== Putative Binding Site ==== | ==== Putative Binding Site ==== | ||
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==== Main Advantages of Developing OspC-based Vaccine ==== | ==== Main Advantages of Developing OspC-based Vaccine ==== | ||
==== Main Problems with Application of OspC-based Vaccine ==== | ==== Main Problems with Application of OspC-based Vaccine ==== | ||
| - | <references/> | + | '''Notes and References'''<references/> |
Revision as of 23:30, 14 August 2012
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Contents |
Outer surface protein C (OspC) of Borrelia burgdorferi
Outer surface protein C (OspC) is one of the major antigens on the surface of the Lyme disease spirochete, Borrelia burgdorferi, along with other outer surface proteins A and B (OspA and OspB, respectively). It greatly differs from OspA and OspB in both structure and function. The uniqueness of OspC is that it comes into play when the pathogen is being transmitted to humans or other mammals. OspC is being produced by Borrelia burgdorferi during a very short time interval when infected ticks start feeding, but its synthesis is known to slow down greatly after transmission to a mammalian host. It was demonstrated that those spirochetes that lack OspC are capable to replicate inside and migrate to the salivary glands of the tick vector but do not infect mammals. [1] Without OspC the spirochetes are believed to be unable to adapt to the environment inside the host. Therefore, OspC is believed to determine virulence of the spirochete to mammals, including humans.
Basic Structure Description
The ospC gene is located on a 27 kb circular plasmid and encodes a lipoprotein of 22–23 kDa.[2] The protein is initially synthesized with an 18-amino-acid-long signal sequence which is removed during processing and lipidation at the amino proximal Cys residue. OspC proteins are highly polymorphic and this variability extends even to strains collected from a single geographical area.[3]
Main Function
Putative Binding Site
Role of OspC in Lyme Disease
OspC-based Vaccine Against Lyme Disease
Main Advantages of Developing OspC-based Vaccine
Main Problems with Application of OspC-based Vaccine
Notes and References- ↑ D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [1]
- ↑ Marconi RT, Samuels DS, Garon CF. Transcriptional analyses and mapping of the ospC gene in Lyme disease spirochetes. J Bacteriol. 1993 Feb;175(4):926-32. PMID:7679385
- ↑ D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [2]
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