1iuc
From Proteopedia
(New page: 200px<br /><applet load="1iuc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iuc, resolution 2.24Å" /> '''Fucose-specific lect...) |
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| - | [[Image:1iuc.jpg|left|200px]]<br /><applet load="1iuc" size=" | + | [[Image:1iuc.jpg|left|200px]]<br /><applet load="1iuc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1iuc, resolution 2.24Å" /> | caption="1iuc, resolution 2.24Å" /> | ||
'''Fucose-specific lectin from Aleuria aurantia with three ligands'''<br /> | '''Fucose-specific lectin from Aleuria aurantia with three ligands'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely | + | Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely used as a specific probe for fucose. Fucosylated sugars often play pivotal roles in many cellular processes. We have determined the crystal structure of AAL at 2.24 A resolution in complex with only three fucose molecules in its five sugar binding sites of a six-fold beta-propeller structure. Very recently, the structure of AAL has been independently determined, showing that all the five binding sites were occupied by fucose molecules [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]. Stabilization of the arginine conformation bound to fucose molecules plays an essential role in generating the difference in the affinity in the five binding sites. Binding models with a couple of saccharides based on biochemical assays suggest that hydrophobic contacts also play important roles in AAL recognizing its ligand. |
==About this Structure== | ==About this Structure== | ||
| - | 1IUC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia] with FUL, FUC and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1IUC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia] with <scene name='pdbligand=FUL:'>FUL</scene>, <scene name='pdbligand=FUC:'>FUC</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IUC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Miki, K.]] | [[Category: Miki, K.]] | ||
[[Category: Nagata, Y.]] | [[Category: Nagata, Y.]] | ||
| - | [[Category: Peapus, D | + | [[Category: Peapus, D H.]] |
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: FUC]] | [[Category: FUC]] | ||
[[Category: FUL]] | [[Category: FUL]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:15:43 2008'' |
Revision as of 11:15, 21 February 2008
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Fucose-specific lectin from Aleuria aurantia with three ligands
Overview
Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely used as a specific probe for fucose. Fucosylated sugars often play pivotal roles in many cellular processes. We have determined the crystal structure of AAL at 2.24 A resolution in complex with only three fucose molecules in its five sugar binding sites of a six-fold beta-propeller structure. Very recently, the structure of AAL has been independently determined, showing that all the five binding sites were occupied by fucose molecules [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]. Stabilization of the arginine conformation bound to fucose molecules plays an essential role in generating the difference in the affinity in the five binding sites. Binding models with a couple of saccharides based on biochemical assays suggest that hydrophobic contacts also play important roles in AAL recognizing its ligand.
About this Structure
1IUC is a Single protein structure of sequence from Aleuria aurantia with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites., Fujihashi M, Peapus DH, Kamiya N, Nagata Y, Miki K, Biochemistry. 2003 Sep 30;42(38):11093-9. PMID:14503859
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