1iv3
From Proteopedia
(New page: 200px<br /><applet load="1iv3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iv3, resolution 1.52Å" /> '''Structure of 2C-Meth...) |
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| - | [[Image:1iv3.gif|left|200px]]<br /><applet load="1iv3" size=" | + | [[Image:1iv3.gif|left|200px]]<br /><applet load="1iv3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1iv3, resolution 1.52Å" /> | caption="1iv3, resolution 1.52Å" /> | ||
'''Structure of 2C-Methyl-D-erythritol-2,4-cyclodiphosphate Synthase (bound form MG atoms)'''<br /> | '''Structure of 2C-Methyl-D-erythritol-2,4-cyclodiphosphate Synthase (bound form MG atoms)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Precursors for isoprenoid synthesis are essential in all organisms. These | + | Precursors for isoprenoid synthesis are essential in all organisms. These compounds are synthesized by one of two known routes: the well characterized mevalonate pathway or a recently discovered non-mevalonate route which is used in many bacteria and human pathogens. Since the second pathway is both vital and unlike any found in humans, enzymes catalysing reactions along this synthetic route are possible drug targets. The structure of one such enzyme from the thermophilic bacterium Thermus thermophilus has been solved to high resolution in the presence of substrate and with a substrate analogue. Enzyme co-crystallized with substrate shows only one product, cytosine monophosphate (CMP), in the active site. At the high resolution of the refinement (1.6 A) the positions and coordination of the magnesium ions in the active site are clearly seen. |
==About this Structure== | ==About this Structure== | ||
| - | 1IV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12] Full crystallographic information is available from [http:// | + | 1IV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IV3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kishida, H.]] | [[Category: Kishida, H.]] | ||
[[Category: Kuzuyama, T.]] | [[Category: Kuzuyama, T.]] | ||
| - | [[Category: Park, S | + | [[Category: Park, S Y.]] |
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Sirouzu, M.]] | [[Category: Sirouzu, M.]] | ||
| - | [[Category: Tame, J | + | [[Category: Tame, J R.H.]] |
[[Category: Terada, T.]] | [[Category: Terada, T.]] | ||
[[Category: Unzai, S.]] | [[Category: Unzai, S.]] | ||
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[[Category: synthase]] | [[Category: synthase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:00 2008'' |
Revision as of 11:16, 21 February 2008
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Structure of 2C-Methyl-D-erythritol-2,4-cyclodiphosphate Synthase (bound form MG atoms)
Overview
Precursors for isoprenoid synthesis are essential in all organisms. These compounds are synthesized by one of two known routes: the well characterized mevalonate pathway or a recently discovered non-mevalonate route which is used in many bacteria and human pathogens. Since the second pathway is both vital and unlike any found in humans, enzymes catalysing reactions along this synthetic route are possible drug targets. The structure of one such enzyme from the thermophilic bacterium Thermus thermophilus has been solved to high resolution in the presence of substrate and with a substrate analogue. Enzyme co-crystallized with substrate shows only one product, cytosine monophosphate (CMP), in the active site. At the high resolution of the refinement (1.6 A) the positions and coordination of the magnesium ions in the active site are clearly seen.
About this Structure
1IV3 is a Single protein structure of sequence from Thermus thermophilus with as ligand. Active as 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, with EC number 4.6.1.12 Full crystallographic information is available from OCA.
Reference
Structure and catalytic mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis., Kishida H, Wada T, Unzai S, Kuzuyama T, Takagi M, Terada T, Shirouzu M, Yokoyama S, Tame JR, Park SY, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):23-31. Epub 2002, Dec 19. PMID:12499535
Page seeded by OCA on Thu Feb 21 13:16:00 2008
Categories: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase | Single protein | Thermus thermophilus | Kishida, H. | Kuzuyama, T. | Park, S Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sirouzu, M. | Tame, J R.H. | Terada, T. | Unzai, S. | Wada, T. | Yokoyama, S. | MG | Isoprenoid | Non-mevalonate | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Synthase
