1j2b
From Proteopedia
(New page: 200px<br /><applet load="1j2b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j2b, resolution 3.30Å" /> '''Crystal Structure Of...) |
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| - | [[Image:1j2b.gif|left|200px]]<br /><applet load="1j2b" size=" | + | [[Image:1j2b.gif|left|200px]]<br /><applet load="1j2b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1j2b, resolution 3.30Å" /> | caption="1j2b, resolution 3.30Å" /> | ||
'''Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)'''<br /> | '''Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transfer RNA (tRNA) canonically has the clover-leaf secondary structure | + | Transfer RNA (tRNA) canonically has the clover-leaf secondary structure with the acceptor, D, anticodon, and T arms, which are folded into the L-shaped tertiary structure. To strengthen the L form, posttranscriptional modifications occur on nucleotides buried within the core, but the modification enzymes are paradoxically inaccessible to them in the L form. In this study, we determined the crystal structure of tRNA bound with archaeosine tRNA-guanine transglycosylase, which modifies G15 of the D arm in the core. The bound tRNA assumes an alternative conformation ("lambda form") drastically different from the L form. All of the D-arm secondary base pairs and the canonical tertiary interactions are disrupted. Furthermore, a helical structure is reorganized, while the rest of the D arm is single stranded and protruded. Consequently, the enzyme precisely locates the exposed G15 in the active site, by counting the nucleotide number from G1 to G15 in the lambda form. |
==About this Structure== | ==About this Structure== | ||
| - | 1J2B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Queuine_tRNA-ribosyltransferase Queuine tRNA-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] Full crystallographic information is available from [http:// | + | 1J2B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Queuine_tRNA-ribosyltransferase Queuine tRNA-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nureki, O.]] | [[Category: Nureki, O.]] | ||
[[Category: Okada, N.]] | [[Category: Okada, N.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:18:09 2008'' |
Revision as of 11:18, 21 February 2008
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Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)
Overview
Transfer RNA (tRNA) canonically has the clover-leaf secondary structure with the acceptor, D, anticodon, and T arms, which are folded into the L-shaped tertiary structure. To strengthen the L form, posttranscriptional modifications occur on nucleotides buried within the core, but the modification enzymes are paradoxically inaccessible to them in the L form. In this study, we determined the crystal structure of tRNA bound with archaeosine tRNA-guanine transglycosylase, which modifies G15 of the D arm in the core. The bound tRNA assumes an alternative conformation ("lambda form") drastically different from the L form. All of the D-arm secondary base pairs and the canonical tertiary interactions are disrupted. Furthermore, a helical structure is reorganized, while the rest of the D arm is single stranded and protruded. Consequently, the enzyme precisely locates the exposed G15 in the active site, by counting the nucleotide number from G1 to G15 in the lambda form.
About this Structure
1J2B is a Single protein structure of sequence from Pyrococcus horikoshii with and as ligands. Active as Queuine tRNA-ribosyltransferase, with EC number 2.4.2.29 Full crystallographic information is available from OCA.
Reference
Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme., Ishitani R, Nureki O, Nameki N, Okada N, Nishimura S, Yokoyama S, Cell. 2003 May 2;113(3):383-94. PMID:12732145
Page seeded by OCA on Thu Feb 21 13:18:09 2008
Categories: Pyrococcus horikoshii | Queuine tRNA-ribosyltransferase | Single protein | Ishitani, R. | Nameki, N. | Nishimura, S. | Nureki, O. | Okada, N. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yokoyama, S. | MG | ZN | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Transferase
