1j6s

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(New page: 200px<br /><applet load="1j6s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j6s, resolution 1.4&Aring;" /> '''Crystal Structure of ...)
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[[Image:1j6s.gif|left|200px]]<br /><applet load="1j6s" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1j6s, resolution 1.4&Aring;" />
caption="1j6s, resolution 1.4&Aring;" />
'''Crystal Structure of an RNA Tetraplex (UGAGGU)4 with A-tetrads, G-tetrads, U-tetrads and G-U octads'''<br />
'''Crystal Structure of an RNA Tetraplex (UGAGGU)4 with A-tetrads, G-tetrads, U-tetrads and G-U octads'''<br />
==Overview==
==Overview==
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Purine-rich regions in DNA and RNA may contain both guanines and adenines, which have various biological functions. Here we report the crystal, structure of an RNA purine-rich fragment containing both guanine and, adenine at 1.4 A resolution. Adenines form an adenine tetrad in the N6-H, em leader N7 conformation. Substitution of an adenine tetrad in the, guanine tetraplex does not change the global conformation but introduces, irregularity in both the hydrogen bonding interaction pattern in the, groove and the metal ion binding pattern in the central cavity of the, tetraplex. The irregularity in groove binding may be critical for specific, binding in tetraplexes. The formation of G-U octads provides a mechanism, for interaction in the groove. Ba(2+) ions prefer to bind guanine tetrads, and adenine tetrads can only be bound by Na(+) ions, illustrating the, binding selectivity of metal ions for the tetraplex.
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Purine-rich regions in DNA and RNA may contain both guanines and adenines, which have various biological functions. Here we report the crystal structure of an RNA purine-rich fragment containing both guanine and adenine at 1.4 A resolution. Adenines form an adenine tetrad in the N6-H em leader N7 conformation. Substitution of an adenine tetrad in the guanine tetraplex does not change the global conformation but introduces irregularity in both the hydrogen bonding interaction pattern in the groove and the metal ion binding pattern in the central cavity of the tetraplex. The irregularity in groove binding may be critical for specific binding in tetraplexes. The formation of G-U octads provides a mechanism for interaction in the groove. Ba(2+) ions prefer to bind guanine tetrads, and adenine tetrads can only be bound by Na(+) ions, illustrating the binding selectivity of metal ions for the tetraplex.
==About this Structure==
==About this Structure==
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1J6S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BA and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J6S OCA].
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1J6S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BA:'>BA</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J6S OCA].
==Reference==
==Reference==
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[[Category: u-tetrads]]
[[Category: u-tetrads]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:11:34 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:24 2008''

Revision as of 11:19, 21 February 2008

Image:1j6s.gif

1j6s, resolution 1.4Å

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Crystal Structure of an RNA Tetraplex (UGAGGU)4 with A-tetrads, G-tetrads, U-tetrads and G-U octads

Overview

Purine-rich regions in DNA and RNA may contain both guanines and adenines, which have various biological functions. Here we report the crystal structure of an RNA purine-rich fragment containing both guanine and adenine at 1.4 A resolution. Adenines form an adenine tetrad in the N6-H em leader N7 conformation. Substitution of an adenine tetrad in the guanine tetraplex does not change the global conformation but introduces irregularity in both the hydrogen bonding interaction pattern in the groove and the metal ion binding pattern in the central cavity of the tetraplex. The irregularity in groove binding may be critical for specific binding in tetraplexes. The formation of G-U octads provides a mechanism for interaction in the groove. Ba(2+) ions prefer to bind guanine tetrads, and adenine tetrads can only be bound by Na(+) ions, illustrating the binding selectivity of metal ions for the tetraplex.

About this Structure

1J6S is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of an RNA purine-rich tetraplex containing adenine tetrads: implications for specific binding in RNA tetraplexes., Pan B, Xiong Y, Shi K, Deng J, Sundaralingam M, Structure. 2003 Jul;11(7):815-23. PMID:12842044

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