1j7n
From Proteopedia
(New page: 200px<br /><applet load="1j7n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j7n, resolution 2.3Å" /> '''Anthrax Toxin Lethal ...) |
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| - | [[Image:1j7n.gif|left|200px]]<br /><applet load="1j7n" size=" | + | [[Image:1j7n.gif|left|200px]]<br /><applet load="1j7n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1j7n, resolution 2.3Å" /> | caption="1j7n, resolution 2.3Å" /> | ||
'''Anthrax Toxin Lethal factor'''<br /> | '''Anthrax Toxin Lethal factor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lethal factor (LF) is a protein (relative molecular mass 90,000) that is | + | Lethal factor (LF) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. It is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near to their amino termini, leading to the inhibition of one or more signalling pathways. Here we describe the crystal structure of LF and its complex with the N terminus of MAPKK-2. LF comprises four domains: domain I binds the membrane-translocating component of anthrax toxin, the protective antigen (PA); domains II, III and IV together create a long deep groove that holds the 16-residue N-terminal tail of MAPKK-2 before cleavage. Domain II resembles the ADP-ribosylating toxin from Bacillus cereus, but the active site has been mutated and recruited to augment substrate recognition. Domain III is inserted into domain II, and seems to have arisen from a repeated duplication of a structural element of domain II. Domain IV is distantly related to the zinc metalloprotease family, and contains the catalytic centre; it also resembles domain I. The structure thus reveals a protein that has evolved through a process of gene duplication, mutation and fusion, into an enzyme with high and unusual specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1J7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with SO4 and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1J7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bienkowska, J.]] | [[Category: Bienkowska, J.]] | ||
| - | [[Category: Collier, R | + | [[Category: Collier, R J.]] |
[[Category: Hanna, P.]] | [[Category: Hanna, P.]] | ||
| - | [[Category: Lacy, D | + | [[Category: Lacy, D B.]] |
| - | [[Category: Leppla, S | + | [[Category: Leppla, S H.]] |
| - | [[Category: Liddington, R | + | [[Category: Liddington, R C.]] |
| - | [[Category: Pannifer, A | + | [[Category: Pannifer, A D.]] |
[[Category: Park, S.]] | [[Category: Park, S.]] | ||
[[Category: Petosa, C.]] | [[Category: Petosa, C.]] | ||
[[Category: Renatus, M.]] | [[Category: Renatus, M.]] | ||
[[Category: Schwarzenbacher, R.]] | [[Category: Schwarzenbacher, R.]] | ||
| - | [[Category: Wong, T | + | [[Category: Wong, T Y.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc metalloprotease]] | [[Category: zinc metalloprotease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:32 2008'' |
Revision as of 11:19, 21 February 2008
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Anthrax Toxin Lethal factor
Overview
Lethal factor (LF) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. It is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near to their amino termini, leading to the inhibition of one or more signalling pathways. Here we describe the crystal structure of LF and its complex with the N terminus of MAPKK-2. LF comprises four domains: domain I binds the membrane-translocating component of anthrax toxin, the protective antigen (PA); domains II, III and IV together create a long deep groove that holds the 16-residue N-terminal tail of MAPKK-2 before cleavage. Domain II resembles the ADP-ribosylating toxin from Bacillus cereus, but the active site has been mutated and recruited to augment substrate recognition. Domain III is inserted into domain II, and seems to have arisen from a repeated duplication of a structural element of domain II. Domain IV is distantly related to the zinc metalloprotease family, and contains the catalytic centre; it also resembles domain I. The structure thus reveals a protein that has evolved through a process of gene duplication, mutation and fusion, into an enzyme with high and unusual specificity.
About this Structure
1J7N is a Single protein structure of sequence from Bacillus anthracis with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the anthrax lethal factor., Pannifer AD, Wong TY, Schwarzenbacher R, Renatus M, Petosa C, Bienkowska J, Lacy DB, Collier RJ, Park S, Leppla SH, Hanna P, Liddington RC, Nature. 2001 Nov 8;414(6860):229-33. PMID:11700563
Page seeded by OCA on Thu Feb 21 13:19:32 2008
Categories: Bacillus anthracis | Single protein | Bienkowska, J. | Collier, R J. | Hanna, P. | Lacy, D B. | Leppla, S H. | Liddington, R C. | Pannifer, A D. | Park, S. | Petosa, C. | Renatus, M. | Schwarzenbacher, R. | Wong, T Y. | SO4 | ZN | Anthrax | Lethal factor | Lethal toxin | Mapkk | Mek | Zinc metalloprotease
