1j83
From Proteopedia
(New page: 200px<br /><applet load="1j83" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j83, resolution 1.70Å" /> '''STRUCTURE OF FAM17 C...) |
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| - | [[Image:1j83.jpg|left|200px]]<br /><applet load="1j83" size=" | + | [[Image:1j83.jpg|left|200px]]<br /><applet load="1j83" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1j83, resolution 1.70Å" /> | caption="1j83, resolution 1.70Å" /> | ||
'''STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS'''<br /> | '''STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the Clostridium cellulovorans | + | The crystal structure of the Clostridium cellulovorans carbohydrate-binding module (CBM) belonging to family 17 has been solved to 1.7 A resolution by multiple anomalous dispersion methods. CBM17 binds to non-crystalline cellulose and soluble beta-1,4-glucans, with a minimal binding requirement of cellotriose and optimal affinity for cellohexaose. The crystal structure of CBM17 complexed with cellotetraose solved at 2.0 A resolution revealed that binding occurs in a cleft on the surface of the molecule involving two tryptophan residues and several charged amino acids. Thermodynamic binding studies and alanine scanning mutagenesis in combination with the cellotetraose complex structure allowed the mapping of the CBM17 binding cleft. In contrast to the binding groove characteristic of family 4 CBMs, family 17 CBMs appear to have a very shallow binding cleft that may be more accessible to cellulose chains in non-crystalline cellulose than the deeper binding clefts of family 4 CBMs. The structural differences in these two modules may reflect non-overlapping binding niches on cellulose surfaces. |
==About this Structure== | ==About this Structure== | ||
| - | 1J83 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http:// | + | 1J83 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J83 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Clostridium cellulovorans]] | [[Category: Clostridium cellulovorans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Boraston, A | + | [[Category: Boraston, A B.]] |
[[Category: Chiu, P.]] | [[Category: Chiu, P.]] | ||
| - | [[Category: Freelove, A | + | [[Category: Freelove, A C.J.]] |
| - | [[Category: Kilburn, D | + | [[Category: Kilburn, D G.]] |
[[Category: Notenboom, V.]] | [[Category: Notenboom, V.]] | ||
| - | [[Category: Rose, D | + | [[Category: Rose, D R.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: carbohydrate binding module]] | [[Category: carbohydrate binding module]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:43 2008'' |
Revision as of 11:19, 21 February 2008
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STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS
Overview
The crystal structure of the Clostridium cellulovorans carbohydrate-binding module (CBM) belonging to family 17 has been solved to 1.7 A resolution by multiple anomalous dispersion methods. CBM17 binds to non-crystalline cellulose and soluble beta-1,4-glucans, with a minimal binding requirement of cellotriose and optimal affinity for cellohexaose. The crystal structure of CBM17 complexed with cellotetraose solved at 2.0 A resolution revealed that binding occurs in a cleft on the surface of the molecule involving two tryptophan residues and several charged amino acids. Thermodynamic binding studies and alanine scanning mutagenesis in combination with the cellotetraose complex structure allowed the mapping of the CBM17 binding cleft. In contrast to the binding groove characteristic of family 4 CBMs, family 17 CBMs appear to have a very shallow binding cleft that may be more accessible to cellulose chains in non-crystalline cellulose than the deeper binding clefts of family 4 CBMs. The structural differences in these two modules may reflect non-overlapping binding niches on cellulose surfaces.
About this Structure
1J83 is a Single protein structure of sequence from Clostridium cellulovorans with as ligand. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study., Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR, J Mol Biol. 2001 Dec 7;314(4):797-806. PMID:11733998
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