1jbo
From Proteopedia
(New page: 200px<br /><applet load="1jbo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jbo, resolution 1.45Å" /> '''The 1.45A Three-Dime...) |
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| - | [[Image:1jbo.jpg|left|200px]]<br /><applet load="1jbo" size=" | + | [[Image:1jbo.jpg|left|200px]]<br /><applet load="1jbo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jbo, resolution 1.45Å" /> | caption="1jbo, resolution 1.45Å" /> | ||
'''The 1.45A Three-Dimensional Structure of c-Phycocyanin from the Thermophylic Cyanobacterium Synechococcus elongatus'''<br /> | '''The 1.45A Three-Dimensional Structure of c-Phycocyanin from the Thermophylic Cyanobacterium Synechococcus elongatus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The conversion of solar radiation to chemical energy by photosynthetic | + | The conversion of solar radiation to chemical energy by photosynthetic organisms provides the primary driving force for life on earth. Light energy is captured by a variety of pigments, usually bound to proteins, which vary with different types of organisms. We report here the 1.45 A resolution three-dimensional structure of one such pigment protein, C-phycocyanin, from Synechococcus elongatus. The structure is at the highest resolution achieved for any such phycobiliprotein. This level of resolution was made possible by implementing a novel crystallization method whereby nucleation is decoupled from subsequent growth, by incubating crystallizing drops for 7h under nucleation conditions and then transferring them to metastable conditions for growth. This is done without touching the crystallization drops throughout the process. |
==About this Structure== | ==About this Structure== | ||
| - | 1JBO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus] with CYC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JBO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus] with <scene name='pdbligand=CYC:'>CYC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Synechococcus elongatus]] | [[Category: Synechococcus elongatus]] | ||
[[Category: Barber, J.]] | [[Category: Barber, J.]] | ||
| - | [[Category: Chayen, N | + | [[Category: Chayen, N E.]] |
[[Category: Nield, J.]] | [[Category: Nield, J.]] | ||
| - | [[Category: Rizkallah, P | + | [[Category: Rizkallah, P J.]] |
[[Category: CYC]] | [[Category: CYC]] | ||
[[Category: photosynthesis]] | [[Category: photosynthesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:01 2008'' |
Revision as of 11:21, 21 February 2008
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The 1.45A Three-Dimensional Structure of c-Phycocyanin from the Thermophylic Cyanobacterium Synechococcus elongatus
Overview
The conversion of solar radiation to chemical energy by photosynthetic organisms provides the primary driving force for life on earth. Light energy is captured by a variety of pigments, usually bound to proteins, which vary with different types of organisms. We report here the 1.45 A resolution three-dimensional structure of one such pigment protein, C-phycocyanin, from Synechococcus elongatus. The structure is at the highest resolution achieved for any such phycobiliprotein. This level of resolution was made possible by implementing a novel crystallization method whereby nucleation is decoupled from subsequent growth, by incubating crystallizing drops for 7h under nucleation conditions and then transferring them to metastable conditions for growth. This is done without touching the crystallization drops throughout the process.
About this Structure
1JBO is a Protein complex structure of sequences from Synechococcus elongatus with as ligand. Full crystallographic information is available from OCA.
Reference
The 1.45 A three-dimensional structure of C-phycocyanin from the thermophilic cyanobacterium Synechococcus elongatus., Nield J, Rizkallah PJ, Barber J, Chayen NE, J Struct Biol. 2003 Feb;141(2):149-55. PMID:12615541
Page seeded by OCA on Thu Feb 21 13:21:01 2008
