2bra

From Proteopedia

Revision as of 12:19, 30 October 2007

STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL

Overview

During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may ... [(full description)]

About this Structure

2BRA is a [Single protein] structure of sequence from [Mus musculus] with CL and FAD as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:16275926

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