2brj
From Proteopedia
(New page: 200px<br /> <applet load="2brj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2brj, resolution 1.50Å" /> '''X-RAY STRUCTURE OF ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BRJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with GOL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.6 5.3.99.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRJ OCA]]. | + | 2BRJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with GOL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Allene-oxide_cyclase Allene-oxide cyclase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.6 5.3.99.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRJ OCA]]. |
==Reference== | ==Reference== | ||
The crystal structure of Arabidopsis thaliana allene oxide cyclase: insights into the oxylipin cyclization reaction., Hofmann E, Zerbe P, Schaller F, Plant Cell. 2006 Nov;18(11):3201-17. Epub 2006 Nov 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17085685 17085685] | The crystal structure of Arabidopsis thaliana allene oxide cyclase: insights into the oxylipin cyclization reaction., Hofmann E, Zerbe P, Schaller F, Plant Cell. 2006 Nov;18(11):3201-17. Epub 2006 Nov 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17085685 17085685] | ||
| + | [[Category: Allene-oxide cyclase]] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transit peptide]] | [[Category: transit peptide]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:24:51 2007'' |
Revision as of 12:20, 30 October 2007
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X-RAY STRUCTURE OF THE ALLENE OXIDE CYCLASE FROM ARABIDOPSIS THALIANA
Overview
We describe the crystallization and structure elucidation of Arabidopsis, thaliana allene oxide cyclase 2 (AOC2), a key enzyme in the biosynthesis, of jasmonates. In a coupled reaction with allene oxide synthase, AOC2, releases the first cyclic and biologically active metabolite, 12-oxo-phytodienoic acid (OPDA). AOC2 (AT3G25770) folds into an, eight-stranded antiparallel beta-barrel with a C-terminal partial helical, extension. The protein forms a hydrophobic binding cavity with two, distinct polar patches. AOC2 is trimeric in crystals, in vitro and in, planta. Based on the observed folding pattern, we assigned AOC2 as a low, molecular weight member of the lipocalin family with enzymatic activity in, plants. We determined the binding position of the competitive inhibitor, vernolic acid (a ... [(full description)]
About this Structure
2BRJ is a [Single protein] structure of sequence from [Arabidopsis thaliana] with GOL as [ligand]. Active as [Allene-oxide cyclase], with EC number [5.3.99.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of Arabidopsis thaliana allene oxide cyclase: insights into the oxylipin cyclization reaction., Hofmann E, Zerbe P, Schaller F, Plant Cell. 2006 Nov;18(11):3201-17. Epub 2006 Nov 3. PMID:17085685
Page seeded by OCA on Tue Oct 30 14:24:51 2007
