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1jes
From Proteopedia
(New page: 200px<br /><applet load="1jes" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jes, resolution 1.5Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size=" | + | [[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jes, resolution 1.5Å" /> | caption="1jes, resolution 1.5Å" /> | ||
'''Crystal Structure of a Copper-Mediated Base Pair in DNA'''<br /> | '''Crystal Structure of a Copper-Mediated Base Pair in DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Stable and selective DNA base pairing by metal coordination was recently | + | Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence. |
==About this Structure== | ==About this Structure== | ||
| - | 1JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JES OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Atwell, S.]] | [[Category: Atwell, S.]] | ||
[[Category: Meggers, E.]] | [[Category: Meggers, E.]] | ||
| - | [[Category: Schultz, P | + | [[Category: Schultz, P G.]] |
[[Category: Spraggon, G.]] | [[Category: Spraggon, G.]] | ||
[[Category: CU]] | [[Category: CU]] | ||
| Line 27: | Line 27: | ||
[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:49 2008'' |
Revision as of 11:21, 21 February 2008
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Crystal Structure of a Copper-Mediated Base Pair in DNA
Overview
Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.
About this Structure
1JES is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a copper-mediated base pair in DNA., Atwell S, Meggers E, Spraggon G, Schultz PG, J Am Chem Soc. 2001 Dec 12;123(49):12364-7. PMID:11734038
Page seeded by OCA on Thu Feb 21 13:21:49 2008
Categories: Protein complex | Atwell, S. | Meggers, E. | Schultz, P G. | Spraggon, G. | CU | Copper | Cu | Designed | Dna | Dodecamer | Duplex | Metal | Unnatural | Z-dna
