1jey
From Proteopedia
(New page: 200px<br /> <applet load="1jey" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jey, resolution 2.50Å" /> '''Crystal Structure o...) |
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caption="1jey, resolution 2.50Å" /> | caption="1jey, resolution 2.50Å" /> | ||
'''Crystal Structure of the Ku heterodimer bound to DNA'''<br /> | '''Crystal Structure of the Ku heterodimer bound to DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic | + | The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle two full turns of DNA while encircling only the central 3-4 base pairs (bp). Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. |
==About this Structure== | ==About this Structure== | ||
| - | 1JEY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1JEY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http:// | + | 1JEY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1JEY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JEY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Corpina, R | + | [[Category: Corpina, R A.]] |
[[Category: Goldberg, J.]] | [[Category: Goldberg, J.]] | ||
| - | [[Category: Walker, J | + | [[Category: Walker, J R.]] |
[[Category: alpha/beta domain]] | [[Category: alpha/beta domain]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
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[[Category: sap domain]] | [[Category: sap domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:53 2008'' |
Revision as of 11:21, 21 February 2008
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Crystal Structure of the Ku heterodimer bound to DNA
Overview
The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle two full turns of DNA while encircling only the central 3-4 base pairs (bp). Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation.
About this Structure
1JEY is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1JEY with [DNA Ligase]. Full crystallographic information is available from OCA.
Reference
Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair., Walker JR, Corpina RA, Goldberg J, Nature. 2001 Aug 9;412(6847):607-14. PMID:11493912
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