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1jgg
From Proteopedia
(New page: 200px<br /><applet load="1jgg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgg, resolution 2.Å" /> '''Even-skipped Homeodoma...) |
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| - | [[Image:1jgg.gif|left|200px]]<br /><applet load="1jgg" size=" | + | [[Image:1jgg.gif|left|200px]]<br /><applet load="1jgg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jgg, resolution 2.Å" /> | caption="1jgg, resolution 2.Å" /> | ||
'''Even-skipped Homeodomain Complexed to AT-rich DNA'''<br /> | '''Even-skipped Homeodomain Complexed to AT-rich DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | even-skipped is a homeobox gene important in controlling segment | + | even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 A resolution. The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity. In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases. |
==About this Structure== | ==About this Structure== | ||
| - | 1JGG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1JGG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aggarwal, A | + | [[Category: Aggarwal, A K.]] |
| - | [[Category: Hirsch, J | + | [[Category: Hirsch, J A.]] |
[[Category: even-skipped]] | [[Category: even-skipped]] | ||
[[Category: homeodomain]] | [[Category: homeodomain]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:21 2008'' |
Revision as of 11:22, 21 February 2008
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Even-skipped Homeodomain Complexed to AT-rich DNA
Overview
even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 A resolution. The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity. In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases.
About this Structure
1JGG is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of the even-skipped homeodomain complexed to AT-rich DNA: new perspectives on homeodomain specificity., Hirsch JA, Aggarwal AK, EMBO J. 1995 Dec 15;14(24):6280-91. PMID:8557047
Page seeded by OCA on Thu Feb 21 13:22:21 2008
