2brt
From Proteopedia
(New page: 200px<br /> <applet load="2brt" size="450" color="white" frame="true" align="right" spinBox="true" caption="2brt, resolution 2.20Å" /> '''ANTHOCYANIDIN SYNTH...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BRT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with FE2, AKG and NAR as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.19 1.14.11.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRT OCA]]. | + | 2BRT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with FE2, AKG and NAR as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Leucocyanidin_oxygenase Leucocyanidin oxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.19 1.14.11.19]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRT OCA]]. |
==Reference== | ==Reference== | ||
Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism., Welford RW, Clifton IJ, Turnbull JJ, Wilson SC, Schofield CJ, Org Biomol Chem. 2005 Sep 7;3(17):3117-26. Epub 2005 Aug 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16106293 16106293] | Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism., Welford RW, Clifton IJ, Turnbull JJ, Wilson SC, Schofield CJ, Org Biomol Chem. 2005 Sep 7;3(17):3117-26. Epub 2005 Aug 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16106293 16106293] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Leucocyanidin oxygenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Clifton, I.J.]] | [[Category: Clifton, I.J.]] | ||
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[[Category: vitamin c]] | [[Category: vitamin c]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:25:16 2007'' |
Revision as of 12:20, 30 October 2007
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ANTHOCYANIDIN SYNTHASE FROM ARABIDOPSIS THALIANA COMPLEXED WITH NARINGENIN
Overview
During the biosynthesis of the tricyclic flavonoid natural products in, plants, oxidative modifications to the central C-ring are catalysed by, Fe(ii) and 2-oxoglutarate dependent oxygenases. The reactions catalysed by, three of these enzymes; flavone synthase I, flavonol synthase and, anthocyanidin synthase (ANS), are formally desaturations. In comparison, flavanone 3beta-hydroxylase catalyses hydroxylation at the C-3 pro-R, position of 2S-naringenin. Incubation of ANS with the unnatural substrate, (+/-)-naringenin results in predominantly C-3 hydroxylation to give, cis-dihydrokaempferol as the major product; trans-dihydrokaempferol and, the desaturation product, apigenin are also observed. Labelling studies, have demonstrated that some of the formal desaturation reactions catalysed, by ... [(full description)]
About this Structure
2BRT is a [Single protein] structure of sequence from [Arabidopsis thaliana] with FE2, AKG and NAR as [ligands]. Active as [Leucocyanidin oxygenase], with EC number [1.14.11.19]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism., Welford RW, Clifton IJ, Turnbull JJ, Wilson SC, Schofield CJ, Org Biomol Chem. 2005 Sep 7;3(17):3117-26. Epub 2005 Aug 1. PMID:16106293
Page seeded by OCA on Tue Oct 30 14:25:16 2007
