1jh3
From Proteopedia
(New page: 200px<br /><applet load="1jh3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jh3" /> '''Solution structure of tyrosyl-tRNA synthetas...) |
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| - | [[Image:1jh3.gif|left|200px]]<br /><applet load="1jh3" size=" | + | [[Image:1jh3.gif|left|200px]]<br /><applet load="1jh3" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of tyrosyl-tRNA synthetase C-terminal domain.'''<br /> | '''Solution structure of tyrosyl-tRNA synthetase C-terminal domain.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a recombinant protein, TyrRS(delta4), corresponding to | + | The structure of a recombinant protein, TyrRS(delta4), corresponding to the anticodon arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase, has been solved, and its dynamics have been studied by nuclear magnetic resonance (NMR). It is the first structure described for such a domain of a tyrosyl-tRNA synthetase. It consists of a five-stranded beta sheet, packed against two alpha helices on one side and one alpha helix on the other side. A large part of the domain is structurally similar to other functionally unrelated RNA binding proteins. The basic residues known to be essential for tRNA binding and charging are exposed to the solvent on the same face of the molecule. The structure of TyrRS(delta4), together with previous mutagenesis data, allows one to delineate the region of interaction with tRNATyr. |
==About this Structure== | ==About this Structure== | ||
| - | 1JH3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http:// | + | 1JH3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JH3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gilquin, B.]] | [[Category: Gilquin, B.]] | ||
[[Category: Guez, V.]] | [[Category: Guez, V.]] | ||
| - | [[Category: Guijarro, J | + | [[Category: Guijarro, J I.]] |
[[Category: Pintar, A.]] | [[Category: Pintar, A.]] | ||
[[Category: Prochnicka-Chalufour, A.]] | [[Category: Prochnicka-Chalufour, A.]] | ||
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[[Category: anticodon-arm binding domain]] | [[Category: anticodon-arm binding domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:32 2008'' |
Revision as of 11:22, 21 February 2008
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Solution structure of tyrosyl-tRNA synthetase C-terminal domain.
Overview
The structure of a recombinant protein, TyrRS(delta4), corresponding to the anticodon arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase, has been solved, and its dynamics have been studied by nuclear magnetic resonance (NMR). It is the first structure described for such a domain of a tyrosyl-tRNA synthetase. It consists of a five-stranded beta sheet, packed against two alpha helices on one side and one alpha helix on the other side. A large part of the domain is structurally similar to other functionally unrelated RNA binding proteins. The basic residues known to be essential for tRNA binding and charging are exposed to the solvent on the same face of the molecule. The structure of TyrRS(delta4), together with previous mutagenesis data, allows one to delineate the region of interaction with tRNATyr.
About this Structure
1JH3 is a Single protein structure of sequence from Geobacillus stearothermophilus. Active as Tyrosine--tRNA ligase, with EC number 6.1.1.1 Full crystallographic information is available from OCA.
Reference
Structure and dynamics of the anticodon arm binding domain of Bacillus stearothermophilus Tyrosyl-tRNA synthetase., Guijarro JI, Pintar A, Prochnicka-Chalufour A, Guez V, Gilquin B, Bedouelle H, Delepierre M, Structure. 2002 Mar;10(3):311-7. PMID:12005430
Page seeded by OCA on Thu Feb 21 13:22:32 2008
