1ji8
From Proteopedia
(New page: 200px<br /><applet load="1ji8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ji8" /> '''Solution Structure of Pyrobaculum Aerophilum...) |
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| - | [[Image:1ji8.gif|left|200px]]<br /><applet load="1ji8" size=" | + | [[Image:1ji8.gif|left|200px]]<br /><applet load="1ji8" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of Pyrobaculum Aerophilum DsrC/gamma subunit of dissimilatory sulfite reductase'''<br /> | '''Solution Structure of Pyrobaculum Aerophilum DsrC/gamma subunit of dissimilatory sulfite reductase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of DsrC, an archaeal homologue of the gamma subunit | + | The solution structure of DsrC, an archaeal homologue of the gamma subunit of dissimilatory sulfite reductase, has been determined by NMR spectroscopy. This 12.7-kDa protein from the hyperthermophilic archaeon Pyrobaculum aerophilum adopts a novel fold consisting of an orthogonal helical bundle with a beta hairpin along one side. A portion of the structure resembles the helix-turn-helix DNA-binding motif common in transcriptional regulator proteins. The protein contains two disulfide bonds but remains folded following reduction of the disulfides. DsrC proteins from organisms other than Pyrobaculum species do not contain these disulfide bonds. A conserved cysteine next to the C-terminus, which is not involved in the disulfide bonds, is located on a seven-residue C-terminal arm that is not part of the globular protein and is likely to dynamically sample more than one conformation. |
==About this Structure== | ==About this Structure== | ||
| - | 1JI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http:// | + | 1JI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pyrobaculum aerophilum]] | [[Category: Pyrobaculum aerophilum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cort, J | + | [[Category: Cort, J R.]] |
| - | [[Category: Kennedy, M | + | [[Category: Kennedy, M A.]] |
| - | [[Category: NESG, Northeast | + | [[Category: NESG, Northeast Structural Genomics Consortium.]] |
[[Category: nesg]] | [[Category: nesg]] | ||
[[Category: northeast structural genomics consortium]] | [[Category: northeast structural genomics consortium]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:55 2008'' |
Revision as of 11:22, 21 February 2008
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Solution Structure of Pyrobaculum Aerophilum DsrC/gamma subunit of dissimilatory sulfite reductase
Overview
The solution structure of DsrC, an archaeal homologue of the gamma subunit of dissimilatory sulfite reductase, has been determined by NMR spectroscopy. This 12.7-kDa protein from the hyperthermophilic archaeon Pyrobaculum aerophilum adopts a novel fold consisting of an orthogonal helical bundle with a beta hairpin along one side. A portion of the structure resembles the helix-turn-helix DNA-binding motif common in transcriptional regulator proteins. The protein contains two disulfide bonds but remains folded following reduction of the disulfides. DsrC proteins from organisms other than Pyrobaculum species do not contain these disulfide bonds. A conserved cysteine next to the C-terminus, which is not involved in the disulfide bonds, is located on a seven-residue C-terminal arm that is not part of the globular protein and is likely to dynamically sample more than one conformation.
About this Structure
1JI8 is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
Solution structure of Pyrobaculum aerophilum DsrC, an archaeal homologue of the gamma subunit of dissimilatory sulfite reductase., Cort JR, Mariappan SV, Kim CY, Park MS, Peat TS, Waldo GS, Terwilliger TC, Kennedy MA, Eur J Biochem. 2001 Nov;268(22):5842-50. PMID:11722571
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