2br3
From Proteopedia
(New page: 200px<br /> <applet load="2br3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2br3, resolution 2.79Å" /> '''CMCI-D160 MG'''<br ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BR3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with MG, 15P and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BR3 OCA]]. | + | 2BR3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with MG, 15P and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BR3 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: porin]] | [[Category: porin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:25:36 2007'' |
Revision as of 12:20, 30 October 2007
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CMCI-D160 MG
Overview
Cephamycin C-producing microorganisms use two enzymes to convert, cephalosporins to their 7alpha-methoxy derivatives. Here we report the, X-ray structure of one of these enzymes, CmcI, from Streptomyces, clavuligerus. The polypeptide chain of the enzyme folds into a C-terminal, Rossmann domain and a smaller N-terminal domain, and the molecule packs as, a hexamer in the crystal. The Rossmann domain binds, S-adenosyl-L-methionine (SAM) and the demethylated product, S-adenosyl-L-homocysteine, in a fashion similar to the common binding mode, of this cofactor in SAM-dependent methyltransferases. There is a, magnesium-binding site in the vicinity of the SAM site with a bound, magnesium ion ligated by residues Asp160, Glu186 and Asp187. The expected, cephalosporin binding site near the magnesium ... [(full description)]
About this Structure
2BR3 is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with MG, 15P and PEG as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus., Oster LM, Lester DR, Terwisscha van Scheltinga A, Svenda M, van Lun M, Genereux C, Andersson I, J Mol Biol. 2006 Apr 28;358(2):546-58. Epub 2006 Feb 21. PMID:16527306
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