1jm6
From Proteopedia
(New page: 200px<br /><applet load="1jm6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jm6, resolution 2.50Å" /> '''Pyruvate dehydrogena...) |
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| - | [[Image:1jm6.jpg|left|200px]]<br /><applet load="1jm6" size=" | + | [[Image:1jm6.jpg|left|200px]]<br /><applet load="1jm6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jm6, resolution 2.50Å" /> | caption="1jm6, resolution 2.50Å" /> | ||
'''Pyruvate dehydrogenase kinase, isozyme 2, containing ADP'''<br /> | '''Pyruvate dehydrogenase kinase, isozyme 2, containing ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of mitochondrial pyruvate dehydrogenase kinase isozyme 2 is | + | The structure of mitochondrial pyruvate dehydrogenase kinase isozyme 2 is of interest because it represents a family of serine-specific protein kinases that lack sequence similarity with all other eukaryotic protein kinases. Similarity exists instead with key motifs of prokaryotic histidine protein kinases and a family of eukaryotic ATPases. The 2.5-A crystal structure reported here reveals that pyruvate dehydrogenase kinase isozyme 2 has two domains of about the same size. The N-terminal half is dominated by a bundle of four amphipathic alpha-helices, whereas the C-terminal half is folded into an alpha/beta sandwich that contains the nucleotide-binding site. Analysis of the structure reveals this C-terminal domain to be very similar to the nucleotide-binding domain of bacterial histidine kinases, but the catalytic mechanism appears similar to that of the eukaryotic serine kinases and ATPases. |
==About this Structure== | ==About this Structure== | ||
| - | 1JM6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/[Pyruvate_dehydrogenase_(acetyl-transferring)]_kinase [Pyruvate dehydrogenase (acetyl-transferring)] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.2 2.7.11.2] Full crystallographic information is available from [http:// | + | 1JM6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/[Pyruvate_dehydrogenase_(acetyl-transferring)]_kinase [Pyruvate dehydrogenase (acetyl-transferring)] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.2 2.7.11.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JM6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: [Pyruvate dehydrogenase (acetyl-transferring)] kinase]] | [[Category: [Pyruvate dehydrogenase (acetyl-transferring)] kinase]] | ||
| - | [[Category: Bowker-Kinley, M | + | [[Category: Bowker-Kinley, M M.]] |
| - | [[Category: Hamilton, J | + | [[Category: Hamilton, J A.]] |
| - | [[Category: Harris, R | + | [[Category: Harris, R A.]] |
| - | [[Category: Popov, K | + | [[Category: Popov, K M.]] |
| - | [[Category: Sloan, R | + | [[Category: Sloan, R B.]] |
| - | [[Category: Steussy, C | + | [[Category: Steussy, C N.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: serine kinase]] | [[Category: serine kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:11 2008'' |
Revision as of 11:24, 21 February 2008
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Pyruvate dehydrogenase kinase, isozyme 2, containing ADP
Overview
The structure of mitochondrial pyruvate dehydrogenase kinase isozyme 2 is of interest because it represents a family of serine-specific protein kinases that lack sequence similarity with all other eukaryotic protein kinases. Similarity exists instead with key motifs of prokaryotic histidine protein kinases and a family of eukaryotic ATPases. The 2.5-A crystal structure reported here reveals that pyruvate dehydrogenase kinase isozyme 2 has two domains of about the same size. The N-terminal half is dominated by a bundle of four amphipathic alpha-helices, whereas the C-terminal half is folded into an alpha/beta sandwich that contains the nucleotide-binding site. Analysis of the structure reveals this C-terminal domain to be very similar to the nucleotide-binding domain of bacterial histidine kinases, but the catalytic mechanism appears similar to that of the eukaryotic serine kinases and ATPases.
About this Structure
1JM6 is a Single protein structure of sequence from Rattus norvegicus with and as ligands. Active as [Pyruvate_dehydrogenase_(acetyl-transferring)_kinase [Pyruvate dehydrogenase (acetyl-transferring)] kinase], with EC number 2.7.11.2 Full crystallographic information is available from OCA.
Reference
Structure of pyruvate dehydrogenase kinase. Novel folding pattern for a serine protein kinase., Steussy CN, Popov KM, Bowker-Kinley MM, Sloan RB Jr, Harris RA, Hamilton JA, J Biol Chem. 2001 Oct 5;276(40):37443-50. Epub 2001 Aug 1. PMID:11483605[[Category: [Pyruvate dehydrogenase (acetyl-transferring)] kinase]]
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