1jmt
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes | + | U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch point. The X-ray structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution. The structure reveals a novel protein-protein recognition strategy, in which an atypical RNA recognition motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via reciprocal "tongue-in-groove" tryptophan residues. Complementary biochemical experiments demonstrate that the core U2AF heterodimer binds RNA, and that the interacting tryptophan side chains are essential for U2AF dimerization. Atypical RRMs in other splicing factors may serve as protein-protein interaction motifs elsewhere during spliceosome assembly. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Green, M | + | [[Category: Green, M R.]] |
| - | [[Category: Kielkopf, C | + | [[Category: Kielkopf, C L.]] |
| - | [[Category: Rodionova, N | + | [[Category: Rodionova, N A.]] |
[[Category: HEZ]] | [[Category: HEZ]] | ||
[[Category: peptide recognition]] | [[Category: peptide recognition]] | ||
| Line 24: | Line 24: | ||
[[Category: rrm]] | [[Category: rrm]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:22 2008'' |
Revision as of 11:24, 21 February 2008
|
X-ray Structure of a Core U2AF65/U2AF35 Heterodimer
Overview
U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch point. The X-ray structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution. The structure reveals a novel protein-protein recognition strategy, in which an atypical RNA recognition motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via reciprocal "tongue-in-groove" tryptophan residues. Complementary biochemical experiments demonstrate that the core U2AF heterodimer binds RNA, and that the interacting tryptophan side chains are essential for U2AF dimerization. Atypical RRMs in other splicing factors may serve as protein-protein interaction motifs elsewhere during spliceosome assembly.
About this Structure
1JMT is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer., Kielkopf CL, Rodionova NA, Green MR, Burley SK, Cell. 2001 Sep 7;106(5):595-605. PMID:11551507
Page seeded by OCA on Thu Feb 21 13:24:22 2008
