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1e3d
From Proteopedia
(New page: 200px<br /> <applet load="1e3d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e3d, resolution 1.80Å" /> '''[NIFE] HYDROGENASE ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E3D is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]] with MG, F3S, SF4, FSX, FNE and H2S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E3D OCA]]. | + | 1E3D is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]] with MG, F3S, SF4, FSX, FNE and H2S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: FE1, FE2, NI1 and NI2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E3D OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: molecular modelling]] | [[Category: molecular modelling]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:26:25 2007'' |
Revision as of 12:21, 30 October 2007
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[NIFE] HYDROGENASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774
Overview
The primary and three-dimensional structures of a [NiFe] hydrogenase, isolated from D. desulfitricans ATCC 27774 were determined, by nucleotide, analysis and single-crystal X-ray crystallography. The three-dimensional, structural model was refined to R=0.167 and Rfree=0.223 using data to 1.8, A resolution. Two unique structural features are observed: the [4Fe-4S], cluster nearest the [NiFe] centre has been modified [4Fe-3S-3O] by loss of, one sulfur atom and inclusion of three oxygen atoms; a three-fold disorder, was observed for Cys536 which binds to the nickel atom in the [NiFe], centre. Also, the bridging sulfur atom that caps the active site was found, to have partial occupancy, thus corresponding to a partly activated, enzyme. These structural features may have biological relevance. ... [(full description)]
About this Structure
1E3D is a [Protein complex] structure of sequences from [Desulfovibrio desulfuricans] with MG, F3S, SF4, FSX, FNE and H2S as [ligands]. Structure known Active Sites: FE1, FE2, NI1 and NI2. Full crystallographic information is available from [OCA].
Reference
[NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774: gene sequencing, three-dimensional structure determination and refinement at 1.8 A and modelling studies of its interaction with the tetrahaem cytochrome c3., Matias PM, Soares CM, Saraiva LM, Coelho R, Morais J, Le Gall J, Carrondo MA, J Biol Inorg Chem. 2001 Jan;6(1):63-81. PMID:11191224
Page seeded by OCA on Tue Oct 30 14:26:25 2007
Categories: Desulfovibrio desulfuricans | Protein complex | Carrondo, M.A. | Coelho, R. | Legall, J. | Matias, P.M. | Morais, J. | Saraiva, L.M. | Soares, C.M. | F3S | FNE | FSX | H2S | MG | SF4 | Crystal structure | Electron transfer | Hydrogenase | Molecular modelling
