1jo7
From Proteopedia
(New page: 200px<br /><applet load="1jo7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jo7" /> '''Solution Structure of Influenza A Virus Prom...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jo7.gif|left|200px]]<br /><applet load="1jo7" size=" | + | [[Image:1jo7.gif|left|200px]]<br /><applet load="1jo7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jo7" /> | caption="1jo7" /> | ||
'''Solution Structure of Influenza A Virus Promoter'''<br /> | '''Solution Structure of Influenza A Virus Promoter'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The influenza A virus, a severe pandemic pathogen, has a segmented RNA | + | The influenza A virus, a severe pandemic pathogen, has a segmented RNA genome consisting of eight single-stranded RNA molecules. The 5' and 3' ends of each RNA segment recognized by the influenza A virus RNA-dependent RNA polymerase direct both transcription and replication of the virus's RNA genome. Promoter binding by the viral RNA polymerase and formation of an active open complex are prerequisites for viral replication and proliferation. Here we describe the solution structure of this promoter as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. Our studies show that the viral promoter has a significant dynamic nature and reveal an unusual displacement of an adenosine that forms a novel (A-A) x U motif and a C-A mismatch stacked in a helix. The characterized structural features of the promoter imply that the specificity of polymerase binding results from an internal RNA loop. In addition, an unexpected bending (46 +/- 10 degrees ) near the initiation site suggests the existence of a promoter recognition mechanism similar to that of DNA-dependent RNA polymerase and a possible regulatory function for the terminal structure during open complex formation. |
==About this Structure== | ==About this Structure== | ||
| - | 1JO7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1JO7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JO7 OCA]. |
==Reference== | ==Reference== | ||
Structural features of an influenza virus promoter and their implications for viral RNA synthesis., Bae SH, Cheong HK, Lee JH, Cheong C, Kainosho M, Choi BS, Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10602-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11553808 11553808] | Structural features of an influenza virus promoter and their implications for viral RNA synthesis., Bae SH, Cheong HK, Lee JH, Cheong C, Kainosho M, Choi BS, Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10602-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11553808 11553808] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bae, S | + | [[Category: Bae, S H.]] |
[[Category: Cheong, C.]] | [[Category: Cheong, C.]] | ||
| - | [[Category: Cheong, H | + | [[Category: Cheong, H K.]] |
| - | [[Category: Choi, B | + | [[Category: Choi, B S.]] |
[[Category: Kainosho, M.]] | [[Category: Kainosho, M.]] | ||
| - | [[Category: Lee, J | + | [[Category: Lee, J H.]] |
[[Category: aau ac mismatch]] | [[Category: aau ac mismatch]] | ||
[[Category: bending]] | [[Category: bending]] | ||
| Line 24: | Line 24: | ||
[[Category: uucg]] | [[Category: uucg]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:48 2008'' |
Revision as of 11:24, 21 February 2008
|
Solution Structure of Influenza A Virus Promoter
Overview
The influenza A virus, a severe pandemic pathogen, has a segmented RNA genome consisting of eight single-stranded RNA molecules. The 5' and 3' ends of each RNA segment recognized by the influenza A virus RNA-dependent RNA polymerase direct both transcription and replication of the virus's RNA genome. Promoter binding by the viral RNA polymerase and formation of an active open complex are prerequisites for viral replication and proliferation. Here we describe the solution structure of this promoter as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. Our studies show that the viral promoter has a significant dynamic nature and reveal an unusual displacement of an adenosine that forms a novel (A-A) x U motif and a C-A mismatch stacked in a helix. The characterized structural features of the promoter imply that the specificity of polymerase binding results from an internal RNA loop. In addition, an unexpected bending (46 +/- 10 degrees ) near the initiation site suggests the existence of a promoter recognition mechanism similar to that of DNA-dependent RNA polymerase and a possible regulatory function for the terminal structure during open complex formation.
About this Structure
1JO7 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural features of an influenza virus promoter and their implications for viral RNA synthesis., Bae SH, Cheong HK, Lee JH, Cheong C, Kainosho M, Choi BS, Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10602-7. PMID:11553808
Page seeded by OCA on Thu Feb 21 13:24:48 2008
Categories: Protein complex | Bae, S H. | Cheong, C. | Cheong, H K. | Choi, B S. | Kainosho, M. | Lee, J H. | Aau ac mismatch | Bending | Internal loop | Rna panhandle | Uucg
