1jpz
From Proteopedia
(New page: 200px<br /><applet load="1jpz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jpz, resolution 1.65Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jpz.gif|left|200px]]<br /><applet load="1jpz" size=" | + | [[Image:1jpz.gif|left|200px]]<br /><applet load="1jpz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jpz, resolution 1.65Å" /> | caption="1jpz, resolution 1.65Å" /> | ||
'''Crystal structure of a complex of the heme domain of P450BM-3 with N-Palmitoylglycine'''<br /> | '''Crystal structure of a complex of the heme domain of P450BM-3 with N-Palmitoylglycine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cytochrome P450s constitute a superfamily of enzymes that catalyze the | + | Cytochrome P450s constitute a superfamily of enzymes that catalyze the oxidation of a vast number of structurally and chemically diverse hydrophobic substrates. Herein, we describe the crystal structure of a complex between the bacterial P450BM-3 and the novel substrate N-palmitoylglycine at a resolution of 1.65 A, which reveals previously unrecognizable features of active site reorganization upon substrate binding. N-palmitoylglycine binds with higher affinity than any other known substrate and reacts with a higher turnover number than palmitic acid but with unaltered regiospecificity along the fatty acid moiety. Substrate binding induces conformational changes in distinct regions of the enzyme including part of the I-helix adjacent to the active site. These changes cause the displacement by about 1 A of the pivotal water molecule that ligands the heme iron, resulting in the low-spin to high-spin conversion of the iron. The water molecule is trapped close to the heme group, which allows it to partition between the iron and the new binding site. This partitioning explains the existence of a high-spin-low-spin equilibrium after substrate binding. The close proximity of the water molecule to the heme iron indicates that it may also participate in the proton-transfer cascade that leads to heterolytic bond scission of oxygen in P450BM-3. |
==About this Structure== | ==About this Structure== | ||
| - | 1JPZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with HEM and 140 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] Full crystallographic information is available from [http:// | + | 1JPZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=140:'>140</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JPZ OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Unspecific monooxygenase]] | [[Category: Unspecific monooxygenase]] | ||
| - | [[Category: Haines, D | + | [[Category: Haines, D C.]] |
[[Category: Machius, M.]] | [[Category: Machius, M.]] | ||
| - | [[Category: Peterson, J | + | [[Category: Peterson, J A.]] |
| - | [[Category: Tomchick, D | + | [[Category: Tomchick, D R.]] |
[[Category: 140]] | [[Category: 140]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
| Line 23: | Line 23: | ||
[[Category: protein-substrate complex]] | [[Category: protein-substrate complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:27 2008'' |
Revision as of 11:25, 21 February 2008
|
Crystal structure of a complex of the heme domain of P450BM-3 with N-Palmitoylglycine
Overview
Cytochrome P450s constitute a superfamily of enzymes that catalyze the oxidation of a vast number of structurally and chemically diverse hydrophobic substrates. Herein, we describe the crystal structure of a complex between the bacterial P450BM-3 and the novel substrate N-palmitoylglycine at a resolution of 1.65 A, which reveals previously unrecognizable features of active site reorganization upon substrate binding. N-palmitoylglycine binds with higher affinity than any other known substrate and reacts with a higher turnover number than palmitic acid but with unaltered regiospecificity along the fatty acid moiety. Substrate binding induces conformational changes in distinct regions of the enzyme including part of the I-helix adjacent to the active site. These changes cause the displacement by about 1 A of the pivotal water molecule that ligands the heme iron, resulting in the low-spin to high-spin conversion of the iron. The water molecule is trapped close to the heme group, which allows it to partition between the iron and the new binding site. This partitioning explains the existence of a high-spin-low-spin equilibrium after substrate binding. The close proximity of the water molecule to the heme iron indicates that it may also participate in the proton-transfer cascade that leads to heterolytic bond scission of oxygen in P450BM-3.
About this Structure
1JPZ is a Single protein structure of sequence from Bacillus megaterium with and as ligands. Active as Unspecific monooxygenase, with EC number 1.14.14.1 Full crystallographic information is available from OCA.
Reference
Pivotal role of water in the mechanism of P450BM-3., Haines DC, Tomchick DR, Machius M, Peterson JA, Biochemistry. 2001 Nov 13;40(45):13456-65. PMID:11695892
Page seeded by OCA on Thu Feb 21 13:25:27 2008
