1ump
From Proteopedia
(New page: 200px<br /> <applet load="1ump" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ump, resolution 2.13Å" /> '''GEOMETRY OF TRITERP...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1UMP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]] with C8E and SQA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.17 5.4.99.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UMP OCA]]. | + | 1UMP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]] with C8E and SQA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Squalene--hopene_cyclase Squalene--hopene cyclase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.17 5.4.99.17]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UMP OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Alicyclobacillus acidocaldarius]] | [[Category: Alicyclobacillus acidocaldarius]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Squalene--hopene cyclase]] | ||
[[Category: Balliano, G.]] | [[Category: Balliano, G.]] | ||
[[Category: Reinert, D.J.]] | [[Category: Reinert, D.J.]] | ||
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[[Category: triterpene cyclase]] | [[Category: triterpene cyclase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:26:46 2007'' |
Revision as of 12:22, 30 October 2007
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GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE
Overview
The membrane protein squalene-hopene cyclase was cocrystallized with, 2-azasqualene and analyzed by X-ray diffraction to 2.13 A resolution. The, conformation of this close analog was clearly established, and it agreed, with the common textbook presentation. The bound squalene undergoes only, small conformational changes during the formation of rings A through D, thus requiring no intermediate. However, ring E formation is hindered by, an entropic barrier, which may explain its absence in the steroids. The, structure analysis revealed a mobile region between the active center, cavity and the membrane, which may melt, opening a passage for squalene, and hopene.
About this Structure
1UMP is a [Single protein] structure of sequence from [Alicyclobacillus acidocaldarius] with C8E and SQA as [ligands]. Active as [Squalene--hopene cyclase], with EC number [5.4.99.17]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Conversion of squalene to the pentacarbocyclic hopene., Reinert DJ, Balliano G, Schulz GE, Chem Biol. 2004 Jan;11(1):121-6. PMID:15113001
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