1jr3
From Proteopedia
(New page: 200px<br /><applet load="1jr3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jr3, resolution 2.7Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1jr3.jpg|left|200px]]<br /><applet load="1jr3" size=" | + | [[Image:1jr3.jpg|left|200px]]<br /><applet load="1jr3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jr3, resolution 2.7Å" /> | caption="1jr3, resolution 2.7Å" /> | ||
'''Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III'''<br /> | '''Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic | + | The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta':gamma(3):delta. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the N-terminal ATP binding domains of the gamma motor and the structurally related domains of the delta' stator and the delta wrench. The structure suggests a mechanism by which the gamma complex switches between a closed state, in which the beta-interacting element of delta is hidden by delta', and an open form similar to the crystal structure, in which delta is free to bind to beta. |
==About this Structure== | ==About this Structure== | ||
| - | 1JR3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1JR3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Donnell, M | + | [[Category: Donnell, M O.]] |
[[Category: Jeruzalmi, D.]] | [[Category: Jeruzalmi, D.]] | ||
[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
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[[Category: processivity clamp]] | [[Category: processivity clamp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:47 2008'' |
Revision as of 11:25, 21 February 2008
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Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III
Overview
The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta':gamma(3):delta. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the N-terminal ATP binding domains of the gamma motor and the structurally related domains of the delta' stator and the delta wrench. The structure suggests a mechanism by which the gamma complex switches between a closed state, in which the beta-interacting element of delta is hidden by delta', and an open form similar to the crystal structure, in which delta is free to bind to beta.
About this Structure
1JR3 is a Protein complex structure of sequences from Escherichia coli with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:11525729
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