1jrg
From Proteopedia
(New page: 200px<br /><applet load="1jrg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jrg, resolution 2.1Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1jrg.jpg|left|200px]]<br /><applet load="1jrg" size=" | + | [[Image:1jrg.jpg|left|200px]]<br /><applet load="1jrg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jrg, resolution 2.1Å" /> | caption="1jrg, resolution 2.1Å" /> | ||
'''Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi'''<br /> | '''Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pectate lyase A is a virulence factor secreted by the plant-pathogenic | + | Pectate lyase A is a virulence factor secreted by the plant-pathogenic bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of pectate polymers by a calcium-dependent beta-elimination mechanism. The crystal structure of pectate lyase A from E. chrysanthemi EC16 has been determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral R3 to 2.1 A. The protein structure is compared with two other pectate lyase isoforms from E. chrysanthemi EC16, pectate lyase C and pectate lyase E. Pectate lyase A is unique as it is the only acidic pectate lyase and has end products that are significantly more varied in length in comparison to those of the other four major pectate lyase isozymes. Differences and similarities in polypeptide trace, size and volume of the active-site groove and surface electrostatics are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1JRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http:// | + | 1JRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Doan, C.]] | [[Category: Doan, C.]] | ||
| - | [[Category: Oliver, R | + | [[Category: Oliver, R L.]] |
| - | [[Category: Thomas, L | + | [[Category: Thomas, L M.]] |
| - | [[Category: Yoder, M | + | [[Category: Yoder, M D.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: parallel beta helix beta elimination]] | [[Category: parallel beta helix beta elimination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:53 2008'' |
Revision as of 11:25, 21 February 2008
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Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi
Overview
Pectate lyase A is a virulence factor secreted by the plant-pathogenic bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of pectate polymers by a calcium-dependent beta-elimination mechanism. The crystal structure of pectate lyase A from E. chrysanthemi EC16 has been determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral R3 to 2.1 A. The protein structure is compared with two other pectate lyase isoforms from E. chrysanthemi EC16, pectate lyase C and pectate lyase E. Pectate lyase A is unique as it is the only acidic pectate lyase and has end products that are significantly more varied in length in comparison to those of the other four major pectate lyase isozymes. Differences and similarities in polypeptide trace, size and volume of the active-site groove and surface electrostatics are discussed.
About this Structure
1JRG is a Single protein structure of sequence from Erwinia chrysanthemi with as ligand. Active as Pectate lyase, with EC number 4.2.2.2 Full crystallographic information is available from OCA.
Reference
Structure of pectate lyase A: comparison to other isoforms., Thomas LM, Doan CN, Oliver RL, Yoder MD, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1008-15. Epub, 2002 May 29. PMID:12037303
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