1jrr
From Proteopedia
(New page: 200px<br /> <applet load="1jrr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jrr, resolution 1.60Å" /> '''HUMAN PLASMINOGEN A...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jrr.gif|left|200px]]<br /> | + | [[Image:1jrr.gif|left|200px]]<br /><applet load="1jrr" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1jrr" size=" | + | |
caption="1jrr, resolution 1.60Å" /> | caption="1jrr, resolution 1.60Å" /> | ||
'''HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP'''<br /> | '''HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the serpin, plasminogen activator inhibitor type-2 | + | The structure of the serpin, plasminogen activator inhibitor type-2 (PAI-2), in a complex with a peptide mimicking its reactive center loop (RCL) has been determined at 1.6-A resolution. The structure shows the relaxed state serpin structure with a prominent six-stranded beta-sheet. Clear electron density is seen for all residues in the peptide. The P1 residue of the peptide binds to a well defined pocket at the base of PAI-2 that may be important in determining the specificity of protease inhibition. The stressed-to-relaxed state (S --> R) transition in PAI-2 can be modeled as the relative motion between a quasirigid core domain and a smaller segment comprising helix hF and beta-strands s1A, s2A, and s3A. A comparison of the Ramachandran plots of the stressed and relaxed state PAI-2 structures reveals the location of several hinge regions connecting these two domains. The hinge regions cluster in three locations on the structure, ensuring a cooperative S --> R transition. We hypothesize that the hinge formed by the conserved Gly(206) on beta-strand s3A in the breach region of PAI-2 effects the S --> R transition by altering its backbone torsion angles. This torsional change is due to the binding of the P14 threonine of the RCL to the open breach region of PAI-2. |
==About this Structure== | ==About this Structure== | ||
| - | 1JRR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ACE and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JRR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRR OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Andrews, J | + | [[Category: Andrews, J L.]] |
| - | [[Category: Baker, M | + | [[Category: Baker, M S.]] |
| - | [[Category: Bertram, K | + | [[Category: Bertram, K C.]] |
| - | [[Category: Curmi, P | + | [[Category: Curmi, P M.G.]] |
| - | [[Category: Gould, A | + | [[Category: Gould, A R.]] |
| - | [[Category: Harrop, S | + | [[Category: Harrop, S J.]] |
[[Category: Jankova, L.]] | [[Category: Jankova, L.]] | ||
| - | [[Category: Saunders, D | + | [[Category: Saunders, D N.]] |
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: BME]] | [[Category: BME]] | ||
| Line 27: | Line 26: | ||
[[Category: serpin]] | [[Category: serpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:59 2008'' |
Revision as of 11:26, 21 February 2008
|
HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP
Overview
The structure of the serpin, plasminogen activator inhibitor type-2 (PAI-2), in a complex with a peptide mimicking its reactive center loop (RCL) has been determined at 1.6-A resolution. The structure shows the relaxed state serpin structure with a prominent six-stranded beta-sheet. Clear electron density is seen for all residues in the peptide. The P1 residue of the peptide binds to a well defined pocket at the base of PAI-2 that may be important in determining the specificity of protease inhibition. The stressed-to-relaxed state (S --> R) transition in PAI-2 can be modeled as the relative motion between a quasirigid core domain and a smaller segment comprising helix hF and beta-strands s1A, s2A, and s3A. A comparison of the Ramachandran plots of the stressed and relaxed state PAI-2 structures reveals the location of several hinge regions connecting these two domains. The hinge regions cluster in three locations on the structure, ensuring a cooperative S --> R transition. We hypothesize that the hinge formed by the conserved Gly(206) on beta-strand s3A in the breach region of PAI-2 effects the S --> R transition by altering its backbone torsion angles. This torsional change is due to the binding of the P14 threonine of the RCL to the open breach region of PAI-2.
About this Structure
1JRR is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the complex of plasminogen activator inhibitor 2 with a peptide mimicking the reactive center loop., Jankova L, Harrop SJ, Saunders DN, Andrews JL, Bertram KC, Gould AR, Baker MS, Curmi PM, J Biol Chem. 2001 Nov 16;276(46):43374-82. Epub 2001 Aug 23. PMID:11546761
Page seeded by OCA on Thu Feb 21 13:25:59 2008
Categories: Homo sapiens | Protein complex | Andrews, J L. | Baker, M S. | Bertram, K C. | Curmi, P M.G. | Gould, A R. | Harrop, S J. | Jankova, L. | Saunders, D N. | ACE | BME | Peptide binding | Serpin
