1jru
From Proteopedia
(New page: 200px<br /><applet load="1jru" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jru" /> '''NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (EN...) |
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| - | [[Image:1jru.jpg|left|200px]]<br /><applet load="1jru" size=" | + | [[Image:1jru.jpg|left|200px]]<br /><applet load="1jru" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jru" /> | caption="1jru" /> | ||
'''NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE)'''<br /> | '''NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | p47 is the major protein identified in complex with the cytosolic AAA | + | p47 is the major protein identified in complex with the cytosolic AAA ATPase p97. It functions as an essential cofactor of p97-regulated membrane fusion, which has been suggested to disassemble t-t-SNARE complexes and prepare them for further rounds of membrane fusion. Here, we report the high-resolution NMR structure of the C-terminal domain from p47. It comprises a UBX domain and a 13 residue long structured N-terminal extension. The UBX domain adopts a characteristic ubiquitin fold with a betabetaalphabetabetaalphabeta secondary structure arrangement. Three hydrophobic residues from the N-terminal extension pack closely against a cleft in the UBX domain. We also identify, for the first time, the p97 interaction surface using NMR chemical shift perturbation studies. |
==About this Structure== | ==About this Structure== | ||
| - | 1JRU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 1JRU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Freemont, P | + | [[Category: Freemont, P S.]] |
[[Category: Kondo, H.]] | [[Category: Kondo, H.]] | ||
[[Category: Lally, J.]] | [[Category: Lally, J.]] | ||
| - | [[Category: Matthews, S | + | [[Category: Matthews, S J.]] |
[[Category: Shaw, A.]] | [[Category: Shaw, A.]] | ||
| - | [[Category: Yuan, X | + | [[Category: Yuan, X M.]] |
| - | [[Category: Zhang, X | + | [[Category: Zhang, X D.]] |
[[Category: ubiquitin superfold]] | [[Category: ubiquitin superfold]] | ||
[[Category: ubx]] | [[Category: ubx]] | ||
[[Category: unusual n-terminal feature]] | [[Category: unusual n-terminal feature]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:03 2008'' |
Revision as of 11:26, 21 February 2008
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NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE)
Overview
p47 is the major protein identified in complex with the cytosolic AAA ATPase p97. It functions as an essential cofactor of p97-regulated membrane fusion, which has been suggested to disassemble t-t-SNARE complexes and prepare them for further rounds of membrane fusion. Here, we report the high-resolution NMR structure of the C-terminal domain from p47. It comprises a UBX domain and a 13 residue long structured N-terminal extension. The UBX domain adopts a characteristic ubiquitin fold with a betabetaalphabetabetaalphabeta secondary structure arrangement. Three hydrophobic residues from the N-terminal extension pack closely against a cleft in the UBX domain. We also identify, for the first time, the p97 interaction surface using NMR chemical shift perturbation studies.
About this Structure
1JRU is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly., Yuan X, Shaw A, Zhang X, Kondo H, Lally J, Freemont PS, Matthews S, J Mol Biol. 2001 Aug 10;311(2):255-63. PMID:11478859
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