1qmd
From Proteopedia
(New page: 200px<br /> <applet load="1qmd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qmd, resolution 2.20Å" /> '''CALCIUM BOUND CLOSE...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QMD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]] with ZN and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QMD OCA]]. | + | 1QMD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]] with ZN and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3]]. Structure known Active Sites: CA1, CA2, CA3, CA4, CA5, CA6, ZN1, ZN2, ZN3 and ZN4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QMD OCA]]. |
==Reference== | ==Reference== | ||
Characterisation of the calcium-binding C-terminal domain of Clostridium perfringens alpha-toxin., Naylor CE, Jepson M, Crane DT, Titball RW, Miller J, Basak AK, Bolgiano B, J Mol Biol. 1999 Dec 3;294(3):757-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10610794 10610794] | Characterisation of the calcium-binding C-terminal domain of Clostridium perfringens alpha-toxin., Naylor CE, Jepson M, Crane DT, Titball RW, Miller J, Basak AK, Bolgiano B, J Mol Biol. 1999 Dec 3;294(3):757-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10610794 10610794] | ||
[[Category: Clostridium perfringens]] | [[Category: Clostridium perfringens]] | ||
| + | [[Category: Phospholipase C]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Basak, A.K.]] | [[Category: Basak, A.K.]] | ||
| Line 25: | Line 26: | ||
[[Category: zinc phospholipase c]] | [[Category: zinc phospholipase c]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:27:11 2007'' |
Revision as of 12:22, 30 October 2007
|
CALCIUM BOUND CLOSED FORM ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS
Overview
Alpha-toxin is the key determinant in gas-gangrene. The toxin, a, phospholipase C, cleaves phosphatidylcholine in eukaryotic cell membranes., Calcium ions have been shown to be required for the specific binding of, toxin to membranes prior to phospholipid cleavage. Reported X-ray, crystallographic structures of the toxin show that the C-terminal domain, has a fold that is analogous to the eukaryotic calcium and, membrane-binding C2 domains. We report the binding sites for three calcium, ions that have been identified, by crystallographic methods, in the, C-terminal domain of the protein close to the postulated membrane-binding, surface. The position of these ions at the tip of the domain, and their, function (to facilitate membrane binding) is similar to that of calcium, ions observed ... [(full description)]
About this Structure
1QMD is a [Single protein] structure of sequence from [Clostridium perfringens] with ZN and CA as [ligands]. Active as [Phospholipase C], with EC number [3.1.4.3]. Structure known Active Sites: CA1, CA2, CA3, CA4, CA5, CA6, ZN1, ZN2, ZN3 and ZN4. Full crystallographic information is available from [OCA].
Reference
Characterisation of the calcium-binding C-terminal domain of Clostridium perfringens alpha-toxin., Naylor CE, Jepson M, Crane DT, Titball RW, Miller J, Basak AK, Bolgiano B, J Mol Biol. 1999 Dec 3;294(3):757-70. PMID:10610794
Page seeded by OCA on Tue Oct 30 14:27:11 2007
