1qmq
From Proteopedia
(New page: 200px<br /> <applet load="1qmq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qmq, resolution 1.55Å" /> '''OPTICAL DETECTION O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1QMQ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with ACT, HEM and LRB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QMQ OCA]]. | + | 1QMQ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with ACT, HEM and LRB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Structure known Active Site: HEM. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QMQ OCA]]. |
==Reference== | ==Reference== | ||
Optical detection of cytochrome P450 by sensitizer-linked substrates., Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10557259 10557259] | Optical detection of cytochrome P450 by sensitizer-linked substrates., Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10557259 10557259] | ||
| + | [[Category: Camphor 5-monooxygenase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ru-substrate]] | [[Category: ru-substrate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:27:27 2007'' |
Revision as of 12:22, 30 October 2007
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OPTICAL DETECTION OF CYTOCHROME P450 BY SENSITIZER-LINKED SUBSTRATES
Overview
The ability to detect, characterize, and manipulate specific biomolecules, in complex media is critical for understanding metabolic processes., Particularly important targets are oxygenases (cytochromes P450) involved, in drug metabolism and many disease states, including liver and kidney, dysfunction, neurological disorders, and cancer. We have found that Ru, photosensitizers linked to P450 substrates specifically recognize, submicromolar cytochrome P450(cam) in the presence of other heme proteins., In the P450:Ru-substrate conjugates, energy transfer to the heme, dramatically accelerates the Ru-luminescence decay. The crystal structure, of a P450(cam):Ru-adamantyl complex reveals access to the active center, via a channel whose depth (Ru-Fe distance is 21 A) is virtually the same, as ... [(full description)]
About this Structure
1QMQ is a [Single protein] structure of sequence from [Pseudomonas putida] with ACT, HEM and LRB as [ligands]. Active as [Camphor 5-monooxygenase], with EC number [1.14.15.1]. Structure known Active Site: HEM. Full crystallographic information is available from [OCA].
Reference
Optical detection of cytochrome P450 by sensitizer-linked substrates., Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12987-90. PMID:10557259
Page seeded by OCA on Tue Oct 30 14:27:27 2007
