1juc
From Proteopedia
(New page: 200px<br /><applet load="1juc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1juc, resolution 2.35Å" /> '''Crystal Structure An...) |
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| - | [[Image:1juc.gif|left|200px]]<br /><applet load="1juc" size=" | + | [[Image:1juc.gif|left|200px]]<br /><applet load="1juc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1juc, resolution 2.35Å" /> | caption="1juc, resolution 2.35Å" /> | ||
'''Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG'''<br /> | '''Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA-strand exchange is a vital step in the recombination process, of which | + | DNA-strand exchange is a vital step in the recombination process, of which a key intermediate is the four-way DNA Holliday junction formed transiently in most living organisms. Here, the single-crystal structure at a resolution of 2.35 A of such a DNA junction formed by d(CCGGTACCGG)(2), which has crystallized in a more highly symmetrical packing mode to that previously observed for the same sequence, is presented. In this case, the structure is isomorphous to the mismatch sequence d(CCGGGACCGG)(2), which reveals the roles of both lattice and DNA sequence in determining the junction geometry. The helices cross at the larger angle of 43.0 degrees (the previously observed angle for this sequence was 41.4 degrees) as a right-handed X. No metal cations were observed; the crystals were grown in the presence of only group I counter-cations. |
==About this Structure== | ==About this Structure== | ||
| - | 1JUC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1JUC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUC OCA]. |
==Reference== | ==Reference== | ||
Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?, Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):567-9. Epub 2002, Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11856858 11856858] | Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?, Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):567-9. Epub 2002, Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11856858 11856858] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cardin, C | + | [[Category: Cardin, C J.]] |
| - | [[Category: Gale, B | + | [[Category: Gale, B C.]] |
| - | [[Category: Teixeira, S | + | [[Category: Teixeira, S C.M.]] |
| - | [[Category: Thorpe, J | + | [[Category: Thorpe, J H.]] |
[[Category: holliday junction]] | [[Category: holliday junction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:48 2008'' |
Revision as of 11:26, 21 February 2008
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Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG
Overview
DNA-strand exchange is a vital step in the recombination process, of which a key intermediate is the four-way DNA Holliday junction formed transiently in most living organisms. Here, the single-crystal structure at a resolution of 2.35 A of such a DNA junction formed by d(CCGGTACCGG)(2), which has crystallized in a more highly symmetrical packing mode to that previously observed for the same sequence, is presented. In this case, the structure is isomorphous to the mismatch sequence d(CCGGGACCGG)(2), which reveals the roles of both lattice and DNA sequence in determining the junction geometry. The helices cross at the larger angle of 43.0 degrees (the previously observed angle for this sequence was 41.4 degrees) as a right-handed X. No metal cations were observed; the crystals were grown in the presence of only group I counter-cations.
About this Structure
1JUC is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?, Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):567-9. Epub 2002, Feb 21. PMID:11856858
Page seeded by OCA on Thu Feb 21 13:26:48 2008
