1jvl
From Proteopedia
(New page: 200px<br /><applet load="1jvl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvl, resolution 2.0Å" /> '''Azurin dimer, covalen...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jvl.gif|left|200px]]<br /><applet load="1jvl" size=" | + | [[Image:1jvl.gif|left|200px]]<br /><applet load="1jvl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jvl, resolution 2.0Å" /> | caption="1jvl, resolution 2.0Å" /> | ||
'''Azurin dimer, covalently crosslinked through bis-maleimidomethylether'''<br /> | '''Azurin dimer, covalently crosslinked through bis-maleimidomethylether'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The transfer of electrons between proteins is an essential step in | + | The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers. |
==About this Structure== | ==About this Structure== | ||
| - | 1JVL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU, NI, 144 and OPP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JVL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=144:'>144</scene> and <scene name='pdbligand=OPP:'>OPP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVL OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amsterdam, I | + | [[Category: Amsterdam, I M.C van.]] |
| - | [[Category: Canters, G | + | [[Category: Canters, G W.]] |
[[Category: Cavazzini, D.]] | [[Category: Cavazzini, D.]] | ||
[[Category: Einsle, O.]] | [[Category: Einsle, O.]] | ||
[[Category: Merli, A.]] | [[Category: Merli, A.]] | ||
[[Category: Messerschmidt, A.]] | [[Category: Messerschmidt, A.]] | ||
| - | [[Category: Rossi, G | + | [[Category: Rossi, G L.]] |
[[Category: Ubbink, M.]] | [[Category: Ubbink, M.]] | ||
[[Category: 144]] | [[Category: 144]] | ||
| Line 29: | Line 29: | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:13 2008'' |
Revision as of 11:27, 21 February 2008
|
Azurin dimer, covalently crosslinked through bis-maleimidomethylether
Overview
The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers.
About this Structure
1JVL is a Single protein structure of sequence from Pseudomonas aeruginosa with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Dramatic modulation of electron transfer in protein complexes by crosslinking., van Amsterdam IM, Ubbink M, Einsle O, Messerschmidt A, Merli A, Cavazzini D, Rossi GL, Canters GW, Nat Struct Biol. 2002 Jan;9(1):48-52. PMID:11740504
Page seeded by OCA on Thu Feb 21 13:27:13 2008
