1jvs
From Proteopedia
(New page: 200px<br /><applet load="1jvs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvs, resolution 2.2Å" /> '''Crystal structure of ...) |
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| - | [[Image:1jvs.gif|left|200px]]<br /><applet load="1jvs" size=" | + | [[Image:1jvs.gif|left|200px]]<br /><applet load="1jvs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jvs, resolution 2.2Å" /> | caption="1jvs, resolution 2.2Å" /> | ||
'''Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase; a target enzyme for antimalarial drugs'''<br /> | '''Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase; a target enzyme for antimalarial drugs'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The key enzyme in the nonmevalonate pathway, 1-deoxy-D-xylulose | + | The key enzyme in the nonmevalonate pathway, 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), has been shown to be an effective target of antimalarial drugs. Here we report the crystal structure of DXR complexed with NADPH and a sulfate ion from Escherichia coli at 2.2 A resolution. The structure showed the presence of an extra domain, which is absent from other NADPH-dependent oxidoreductases, in addition to the conformation of catalytic residues and the substrate binding site. A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1JVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] Full crystallographic information is available from [http:// | + | 1JVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVS OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding., Yajima S, Nonaka T, Kuzuyama T, Seto H, Ohsawa K, J Biochem | + | Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding., Yajima S, Nonaka T, Kuzuyama T, Seto H, Ohsawa K, J Biochem. 2002 Mar;131(3):313-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11872159 11872159] |
[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]] | [[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: reductoisomerase]] | [[Category: reductoisomerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:17 2008'' |
Revision as of 11:27, 21 February 2008
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Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase; a target enzyme for antimalarial drugs
Overview
The key enzyme in the nonmevalonate pathway, 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), has been shown to be an effective target of antimalarial drugs. Here we report the crystal structure of DXR complexed with NADPH and a sulfate ion from Escherichia coli at 2.2 A resolution. The structure showed the presence of an extra domain, which is absent from other NADPH-dependent oxidoreductases, in addition to the conformation of catalytic residues and the substrate binding site. A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity.
About this Structure
1JVS is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as 1-deoxy-D-xylulose-5-phosphate reductoisomerase, with EC number 1.1.1.267 Full crystallographic information is available from OCA.
Reference
Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding., Yajima S, Nonaka T, Kuzuyama T, Seto H, Ohsawa K, J Biochem. 2002 Mar;131(3):313-7. PMID:11872159
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