1jvx
From Proteopedia
(New page: 200px<br /><applet load="1jvx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvx, resolution 2.50Å" /> '''Maltodextrin-binding...) |
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| - | [[Image:1jvx.gif|left|200px]]<br /><applet load="1jvx" size=" | + | [[Image:1jvx.gif|left|200px]]<br /><applet load="1jvx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jvx, resolution 2.50Å" /> | caption="1jvx, resolution 2.50Å" /> | ||
'''Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal'''<br /> | '''Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cysteine substitutions were engineered on the surface of maltose binding | + | Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material. |
==About this Structure== | ==About this Structure== | ||
| - | 1JVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MAL:'>MAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bell, J | + | [[Category: Bell, J A.]] |
| - | [[Category: Iyer, G | + | [[Category: Iyer, G H.]] |
| - | [[Category: Przybycien, T | + | [[Category: Przybycien, T A.]] |
| - | [[Category: Samsonoff, W | + | [[Category: Samsonoff, W A.]] |
[[Category: Srinivasan, U.]] | [[Category: Srinivasan, U.]] | ||
[[Category: MAL]] | [[Category: MAL]] | ||
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[[Category: intermolecular]] | [[Category: intermolecular]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:20 2008'' |
Revision as of 11:27, 21 February 2008
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Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal
Overview
Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material.
About this Structure
1JVX is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystine: fibrous biomolecular material from protein crystals cross-linked in a specific geometry., Srinivasan U, Iyer GH, Przybycien TA, Samsonoff WA, Bell JA, Protein Eng. 2002 Nov;15(11):895-902. PMID:12538909
Page seeded by OCA on Thu Feb 21 13:27:20 2008
