1jw6
From Proteopedia
(New page: 200px<br /><applet load="1jw6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jw6, resolution 1.93Å" /> '''Crystal Structure of...) |
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| - | [[Image:1jw6.gif|left|200px]]<br /><applet load="1jw6" size=" | + | [[Image:1jw6.gif|left|200px]]<br /><applet load="1jw6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jw6, resolution 1.93Å" /> | caption="1jw6, resolution 1.93Å" /> | ||
'''Crystal Structure of the Complex of Concanavalin A and Hexapeptide'''<br /> | '''Crystal Structure of the Complex of Concanavalin A and Hexapeptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray structure analysis of a cross-linked crystal of concanavalin A | + | The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with a hexapeptide molecule as a probe molecule showed an electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the hexapeptide complex was refined at 1.93-A resolution, to an R-factor of 19% (Rfree factor of 25%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residues of concanavalin A, one manganese ion, one calcium ion, 95 water molecules, one glutaraldehyde molecule, one isopropanol molecule, and one hexapeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in the mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution. |
==About this Structure== | ==About this Structure== | ||
| - | 1JW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with MN, CA, PTD and IPA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=PTD:'>PTD</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: complex with hexapeptide]] | [[Category: complex with hexapeptide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:28 2008'' |
Revision as of 11:27, 21 February 2008
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Crystal Structure of the Complex of Concanavalin A and Hexapeptide
Overview
The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with a hexapeptide molecule as a probe molecule showed an electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the hexapeptide complex was refined at 1.93-A resolution, to an R-factor of 19% (Rfree factor of 25%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residues of concanavalin A, one manganese ion, one calcium ion, 95 water molecules, one glutaraldehyde molecule, one isopropanol molecule, and one hexapeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in the mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution.
About this Structure
1JW6 is a Single protein structure of sequence from Canavalia ensiformis with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the complex of concanavalin A and hexapeptide., Zhang Z, Qian M, Huang Q, Jia Y, Tang Y, Wang K, Cui D, Li M, J Protein Chem. 2001 Jul;20(5):423-9. PMID:11732694
Page seeded by OCA on Thu Feb 21 13:27:28 2008
Categories: Canavalia ensiformis | Single protein | Huang, Q. | Jia, Y. | Qian, M. | Tang, Y. | Zhang, Z. | CA | IPA | MN | PTD | Complex with hexapeptide
