1jxs
From Proteopedia
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'''Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor'''<br /> | '''Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Interleukin enhancer binding factor (ILF) binds to the interleukin-2 | + | Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1JXS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1JXS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, S | + | [[Category: Chen, S H.]] |
| - | [[Category: Chen, S | + | [[Category: Chen, S W.]] |
| - | [[Category: Chuang, W | + | [[Category: Chuang, W J.]] |
| - | [[Category: Hsieh, Y | + | [[Category: Hsieh, Y H.]] |
| - | [[Category: Jeng, W | + | [[Category: Jeng, W Y.]] |
[[Category: Li, C.]] | [[Category: Li, C.]] | ||
| - | [[Category: Liu, P | + | [[Category: Liu, P P.]] |
[[Category: dna-binding domain]] | [[Category: dna-binding domain]] | ||
[[Category: winged helix]] | [[Category: winged helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:52 2008'' |
Revision as of 11:27, 21 February 2008
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Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor
Overview
Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.
About this Structure
1JXS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)., Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ, Proteins. 2002 Dec 1;49(4):543-53. PMID:12402362
Page seeded by OCA on Thu Feb 21 13:27:52 2008
