1k09
From Proteopedia
(New page: 200px<br /><applet load="1k09" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k09" /> '''Solution structure of BetaCore, A Designed W...) |
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'''Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein'''<br /> | '''Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | BetaCore is a designed approximately 50-residue protein in which two | + | BetaCore is a designed approximately 50-residue protein in which two BPTI-derived core modules, CM I and CM II, are connected by a 22-atom cross-link. At low temperature and pH 3, homo- and heteronuclear NMR data report a dominant folded ('f') conformation with well-dispersed chemical shifts, i, i+1 periodicity, numerous long-range NOEs, and slowed amide hydrogen isotope exchange patterns that is a four-stranded antiparallel beta-sheet with nonsymmetrical and specific association of CM I and CM II. BetaCore 'f' conformations undergo reversible, global, moderately cooperative, non-two-state thermal transitions to an equilibrium ensemble of unfolded 'u' conformations. There is a significant energy barrier between 'f' and 'u' conformations. This is the first designed four-stranded antiparallel beta-sheet that folds in water. |
==About this Structure== | ==About this Structure== | ||
| - | 1K09 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1K09 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K09 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: four-stranded antiparallel beta-sheet]] | [[Category: four-stranded antiparallel beta-sheet]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:42 2008'' |
Revision as of 11:28, 21 February 2008
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Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein
Overview
BetaCore is a designed approximately 50-residue protein in which two BPTI-derived core modules, CM I and CM II, are connected by a 22-atom cross-link. At low temperature and pH 3, homo- and heteronuclear NMR data report a dominant folded ('f') conformation with well-dispersed chemical shifts, i, i+1 periodicity, numerous long-range NOEs, and slowed amide hydrogen isotope exchange patterns that is a four-stranded antiparallel beta-sheet with nonsymmetrical and specific association of CM I and CM II. BetaCore 'f' conformations undergo reversible, global, moderately cooperative, non-two-state thermal transitions to an equilibrium ensemble of unfolded 'u' conformations. There is a significant energy barrier between 'f' and 'u' conformations. This is the first designed four-stranded antiparallel beta-sheet that folds in water.
About this Structure
1K09 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
BetaCore, a designed water soluble four-stranded antiparallel beta-sheet protein., Carulla N, Woodward C, Barany G, Protein Sci. 2002 Jun;11(6):1539-51. PMID:12021452
Page seeded by OCA on Thu Feb 21 13:28:42 2008
