1k0h
From Proteopedia
(New page: 200px<br /><applet load="1k0h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k0h" /> '''Solution structure of bacteriophage lambda g...) |
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| - | [[Image:1k0h.gif|left|200px]]<br /><applet load="1k0h" size=" | + | [[Image:1k0h.gif|left|200px]]<br /><applet load="1k0h" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of bacteriophage lambda gpFII'''<br /> | '''Solution structure of bacteriophage lambda gpFII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The bacteriophage lambda FII protein (gpFII) is a 117 residue structural | + | The bacteriophage lambda FII protein (gpFII) is a 117 residue structural protein found in the phage particle that is required for the joining of phage heads and tails at the last step of morphogenesis. We have performed biophysical experiments to show that gpFII is stable, monomeric, and reversibly folded. We have also determined the atomic resolution structure of gpFII using NMR spectroscopy. gpFII is shown to possess a novel fold consisting of seven beta-strands and a short alpha-helix. It also displays two large unstructured regions at the N terminus (residues 1-24) and in a large loop near the middle of the protein (residues 46-62). We speculate that these unstructured regions become structured when gpFII assembles into the phage particle, and that these conformational changes play an important role in regulating the assembly pathway. Alignment of the gpFII sequence with those of homologues from other lambdoid phages has allowed us to putatively identify distinct surfaces on the gpFII structure that mediate binding to the phage head and tail. |
==About this Structure== | ==About this Structure== | ||
| - | 1K0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http:// | + | 1K0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Davidson, A | + | [[Category: Davidson, A R.]] |
[[Category: Gold, M.]] | [[Category: Gold, M.]] | ||
| - | [[Category: Maxwell, K | + | [[Category: Maxwell, K L.]] |
| - | [[Category: Yee, A | + | [[Category: Yee, A A.]] |
[[Category: twisted beta-sandwich]] | [[Category: twisted beta-sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:44 2008'' |
Revision as of 11:28, 21 February 2008
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Solution structure of bacteriophage lambda gpFII
Overview
The bacteriophage lambda FII protein (gpFII) is a 117 residue structural protein found in the phage particle that is required for the joining of phage heads and tails at the last step of morphogenesis. We have performed biophysical experiments to show that gpFII is stable, monomeric, and reversibly folded. We have also determined the atomic resolution structure of gpFII using NMR spectroscopy. gpFII is shown to possess a novel fold consisting of seven beta-strands and a short alpha-helix. It also displays two large unstructured regions at the N terminus (residues 1-24) and in a large loop near the middle of the protein (residues 46-62). We speculate that these unstructured regions become structured when gpFII assembles into the phage particle, and that these conformational changes play an important role in regulating the assembly pathway. Alignment of the gpFII sequence with those of homologues from other lambdoid phages has allowed us to putatively identify distinct surfaces on the gpFII structure that mediate binding to the phage head and tail.
About this Structure
1K0H is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
The solution structure of the bacteriophage lambda head-tail joining protein, gpFII., Maxwell KL, Yee AA, Arrowsmith CH, Gold M, Davidson AR, J Mol Biol. 2002 May 17;318(5):1395-404. PMID:12083526
Page seeded by OCA on Thu Feb 21 13:28:44 2008
